Evidence map›Paper›PMID 40445532›Full record

ArticleEuropean archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry2025

A proteomics approach to understand the hypomineralisation of second primary molars (HSPM). In-vitro study.

A Singh, A Goyal, M Luthra-Guptasarma, A Kapur, M Jaiswal, S Madan

Abstract read
PubMed Publisher
In one paragraph

Article in European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

A SinghUnit of Pedodontics, Oral Health Sciences Center, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India.
A GoyalUnit of Pedodontics, Oral Health Sciences Center, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India. ashimapgi@yahoo.in.
M Luthra-GuptasarmaDepartment of Immunopathology, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India. guptasarma.manni@pgimer.edu.in.ORCID http://orcid.org/0000-0002-2740-887X
A KapurUnit of Pedodontics, Oral Health Sciences Center, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India.
M JaiswalUnit of Pedodontics, Oral Health Sciences Center, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India.
S MadanDepartment of Immunopathology, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India.

Funding

PGIMER IM/305/21-03-23-0832
6 · The paper itself

Abstract

purposeThe purpose of this study was a proteomic investigation of test enamel of HSPM vs the control enamel of Second Primary Molars, to gain an insight into the possible mechanism of pathogenesis of hypomineralisation.

methodsEnamel sample blocks were sectioned and processed for label-free quantitation of proteins by LC-MS/MS, followed by bioinformatics analyses of differentially expressed proteins (DEP); two DEPs were validated by western/dot blot.

resultsThere was a significant elevation of total number of proteins in the HSPM samples as compared to the control enamel. A significant elevation of proteins such as protein LEG1 homolog, Odontogenesis-associated phosphoprotein, Leucocyte elastase inhibitor, Olfactomedin-like protein 3 and albumin were observed in HSPM-affected enamel, along with upregulation of calcium-binding proteins and immunoglobulins. Evaluation of signalling pathways contributed by the upregulated proteins in HSPM enamel samples was suggestive of increased levels of different components of the immune (defence) system such as raised antibodies (immunoglobulins level), complement coagulation cascade activation, neutrophil and platelet degranulation as well as defence response to bacteria/ fungus.

conclusionsThe study results suggest the possibility of an underlying infectious/inflammatory aetiology responsible for hypomineralisation of primary enamel in these patients. Such a proteomics approach is useful to guide preventive interventions for HSPM.

Indexed as

Dental Enamel HypoplasiaMolarProteomicsTooth, DeciduousBlotting, WesternChromatography, LiquidDental Enamel ProteinsHumansIn Vitro TechniquesTandem Mass SpectrometryDental Enamel ProteinsHSPMImmunoglobulinsODAPHOLFML3Primary molarsProteomics

Identifiers

PMID40445532

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.