Evidence map›Paper›PMID 40446196›Full record

ArticleDevelopment (Cambridge, England)2025

WNT signaling coordinately controls mouse limb bud outgrowth and establishment of the digit-interdigit pattern.

Jonas Malkmus, Angela Morabito, Lucille Lopez-Delisle, Laura Avino-Esteban, Alexandre Mayran, Aimee Zuniga, James Sharpe, Rolf Zeller, Rushikesh Sheth

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Evidence for Fgf and Wnt regulation ofFrontiers in cell and developmental biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jonas MalkmusDevelopmental Genetics, Department of Biomedicine, University of Basel, CH4054 Basel, Switzerland.
Angela MorabitoDevelopmental Genetics, Department of Biomedicine, University of Basel, CH4054 Basel, Switzerland.
Lucille Lopez-DelisleSchool of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), CH 1015 Lausanne, Switzerland.
Laura Avino-EstebanMulticellular Systems Biology, European Molecular Biology Laboratory (EMBL-Barcelona), Barcelona 08003, Spain.
Alexandre MayranSchool of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), CH 1015 Lausanne, Switzerland.
Aimee ZunigaDevelopmental Genetics, Department of Biomedicine, University of Basel, CH4054 Basel, Switzerland.
James SharpeMulticellular Systems Biology, European Molecular Biology Laboratory (EMBL-Barcelona), Barcelona 08003, Spain.
Rolf ZellerDevelopmental Genetics, Department of Biomedicine, University of Basel, CH4054 Basel, Switzerland.ORCID 0000-0002-3186-7403
Rushikesh ShethDevelopmental Genetics, Department of Biomedicine, University of Basel, CH4054 Basel, Switzerland.

Funding

European Molecular Biology LaboratoryEuropean Research Council 588029European Research Council 695032European Research Council INTEGRAL ERC-2015-AdGHORIZON EUROPE European Research Council INTEGRAL ERC-2015-AdGHuman Frontier Science Program LT000032/2019-LSchweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung 310030_166685BSchweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung 310030_184734Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung 310030_196868Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung 310030_207824Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung 407940_206405Schweizerischer Nationalfonds zur Furderung der Wissenschaftlichen Forschung 310030_166685BSchweizerischer Nationalfonds zur Furderung der Wissenschaftlichen Forschung 310030_184734Schweizerischer Nationalfonds zur Furderung der Wissenschaftlichen Forschung 310030_196868Schweizerischer Nationalfonds zur Furderung der Wissenschaftlichen Forschung 310030_207824Schweizerischer Nationalfonds zur Furderung der Wissenschaftlichen Forschung 407940_206405Universität Basel
6 · The paper itself

Abstract

Self-organization, such as the emergence of a pattern from a homogenous state, is a fascinating property of biological systems. Early limb bud outgrowth and patterning in mice are controlled by a robust and self-regulatory signaling system, and initiation of the periodic digit-interdigit pattern appears to be under the control of a self-regulatory Turing system. Previous studies established the requirement of WNT and BMP signaling for both early limb bud and digit-interdigit morphogenesis, but the molecular changes underlying the transition from early limb bud signaling to the digit-interdigit patterning system remained unknown. Here, we have used small molecule inhibitors to rapidly but transiently block WNT signaling to identify the early transcriptional targets that are altered during disruption and recovery of limb bud and digit development. Together, this study highlights the overarching role of WNT signaling in controlling early limb bud outgrowth and patterning, and the establishment of the periodic digit-interdigit pattern. Finally, the transient WNT signaling disruption approach reveals the plasticity and robustness of these self-organizing limb bud- and digit-patterning systems.

Indexed as

Body PatterningLimb BudsWnt ProteinsWnt Signaling PathwayAnimalsExtremitiesGene Expression Regulation, DevelopmentalMiceWnt ProteinsDigit-interdigit patternLimb bud developmentPlasticityRobustnessSelf-regulationSignaling systemWNT/β-catenin

Identifiers

PMID40446196
PMCPMC12188246

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.