Evidence map›Paper›PMID 40448189›Full record

ArticleCell & bioscience2025

Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency.

Zhuran Zhao, Xi Chen, Shan Wang, Min Fu, Huan Shen, Jiayu Li, Jun Xu, Jiong Qin, Cheng Shi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhuran Zhao *Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China.
Xi Chen *Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China.
Shan Wang *Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China.
Min FuReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China.
Huan ShenReproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China.
Jiayu LiDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing, 100191, China.
Jun XuDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing, 100191, China.
Jiong Qin *Department of Pediatrics, Peking University People's Hospital, Beijing, 100044, China. qinjiong@pkuph.edu.cn.
Cheng Shi *Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University People's Hospital, Peking University, Beijing, 100044, China. chengshi@bjmu.edu.cn.

Funding

National Natural Science Foundation of China 82301872
6 · The paper itself

Abstract

Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, we report an optimized, highly efficient protocol by utilizing chemically defined and xeno-free media for the derivation of human extended pluripotent stem cell lines from discarded blastocysts. With this method, we successfully isolated hEPS cell lines from discarded blastocysts with an efficiency of 46%. Chemically defined and xeno-free media simplified the process of hEPS cell isolation with a higher survival rate of cell aggregation passaging from outgrowth. To our knowledge, this is the first report of hEPS cells being efficiently derived from discarded blastocysts under chemically defined and xeno-free conditions.

Indexed as

Cell lines establishment efficiencyChemically definedEmbryonic and extraembryonic differentiation capacityHuman extended pluripotent stem cellsXeno-free

Identifiers

PMID40448189
PMCPMC12123846

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.