Evidence map›Paper›PMID 40448398›Full record

ArticleActa physiologica (Oxford, England)2025

Branched-Chain Amino Acids and Di-Alanine Supplementation Attenuates Muscle Atrophy in a Murine Model of Cancer Cachexia.

Mayra Colardo, Noemi Martella, Michela Varone, Daniele Pensabene, Giuseppina Caretti, Gianluca Bianchini, Andrea Aramini, Marco Segatto

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In one paragraph

Article in Acta physiologica (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mayra ColardoDepartment of Biosciences and Territory, University of Molise, Pesche, Italy.
Noemi MartellaDepartment of Biosciences and Territory, University of Molise, Pesche, Italy.
Michela VaroneDepartment of Biosciences and Territory, University of Molise, Pesche, Italy.
Daniele PensabeneDepartment of Biosciences and Territory, University of Molise, Pesche, Italy.
Giuseppina CarettiDepartment of Biosciences, University of Milan, Milan, Italy.
Gianluca BianchiniResearch & Early Development, Dompé farmaceutici S.p.A., L'Aquila, Italy.
Andrea AraminiResearch & Early Development, Dompé farmaceutici S.p.A., L'Aquila, Italy.
Marco SegattoDepartment of Biosciences and Territory, University of Molise, Pesche, Italy.ORCID https://orcid.org/0000-0003-0465-9470

Funding

AIRC-IG21353Fondo per la Crescita Sostenibile-Bando "HORIZON 2020" PON I&C 2014-2020 (FARMIDIAB)Funds for Departmental Research 2022-University of Molise
6 · The paper itself

Abstract

aimCancer cachexia is a severe metabolic disorder leading to skeletal muscle atrophy. Muscle wasting is a major clinical problem in cachectic patients, as it limits the efficacy of chemotherapeutic treatments and worsens quality of life. Nutritional support based on branched-chain amino acids (BCAA) has been shown to be a promising approach to counteract cachexia-induced muscle atrophy, but its efficacy is still debated. Furthermore, the putative role of di-alanine (Di-Ala) supplementation has yet to be evaluated. The present study therefore sought to assess whether BCAA supplementation, alone or in combination with a Di-Ala peptide, could attenuate muscle wasting in a preclinical model of cancer cachexia.

methodsTo this end, C26 tumor-bearing mice were administered BCAA supplementation, with or without Di-Ala. Body and muscle weights, as well as molecular, biochemical, and morphological analysis, were carried out to characterize prospective changes of markers involved in cachexia and muscle atrophy.

resultsThe main findings revealed that BCAA supplementation effectively prevented body weight loss and muscle atrophy. Of note, Di-Ala significantly amplified the effects of BCAA. These phenomena were found to be mediated by the suppression of pathways involved in protein catabolism.

conclusionsCollectively, these results highlight that innovative formulations containing Di-Ala, capable of increasing BCAA bioavailability, may be efficacious in counteracting muscle atrophy, especially during mild-to-moderate cancer cachexia.

Indexed as

Amino Acids, Branched-ChainCachexiaMuscular AtrophyNeoplasmsAnimalsDietary SupplementsDisease Models, AnimalMaleMiceMuscle, SkeletalAmino Acids, Branched-ChainBCAAdietary supplementsmuscle wastingnutraceuticalsprotein catabolismskeletal muscle

Identifiers

PMID40448398
PMCPMC12125566

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.