Evidence map›Paper›PMID 40456366›Full record

ArticleVirus research2025

Preparation and pharmacokinetic evaluation of Staphylococcus phage COP-80B for treatment of periprosthetic joint infections in a mouse model.

Vida Štilec, Monika Marušić, Nika Janež, Urban Bezeljak, Lucija Rebula, Maja Leskovec, Rihard Trebše, Simon Horvat, Matjaž Peterka

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Vancomycin and phage COP-80B combination therapy for Staphylococcus epidermidis periprosthetic joint infections: a preclinical mouse study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Vida ŠtilecCOBIK, Mirce 21, 5270 Ajdovščina, Slovenia; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia. Electronic address: vida.stilec@cobik.si.
Monika MarušićCOBIK, Mirce 21, 5270 Ajdovščina, Slovenia.
Nika JanežCOBIK, Mirce 21, 5270 Ajdovščina, Slovenia.
Urban BezeljakCOBIK, Mirce 21, 5270 Ajdovščina, Slovenia; Sferogen d.o.o, Vipavska cesta 2c, 5270 Ajdovščina, Slovenia.
Lucija RebulaSartorius BIA Separations d.o.o., Mirce 21, 5270 Ajdovščina, Slovenia.
Maja LeskovecSartorius BIA Separations d.o.o., Mirce 21, 5270 Ajdovščina, Slovenia.
Rihard TrebšeValdoltra Orthopaedic Hospital, Jadranska cesta 31, 6280 Ankaran, Slovenia; Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.
Simon HorvatDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.
Matjaž PeterkaCOBIK, Mirce 21, 5270 Ajdovščina, Slovenia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phage therapy has recently attracted significant attention as a potential treatment for periprosthetic joint infections, yielding promising outcomes in several compassionate use cases. The absence of standardized treatment protocols is partly attributable to insufficient pharmacokinetic data regarding relevant phage administration routes and dosages. Another neglected aspect is the scalable manufacturing of pharmaceutical-grade phage preparations for preclinical testing. In this study, we address both challenges and present a scalable phage production process for the Staphylococcus epidermidis-specific phage COP-80B We prepared a highly purified phage suspension, as verified through qPCR, HPLC, NTA and short-read sequencing, which was used in a preclinical pharmacokinetic study in an uninfected mice model. Using a plaque assay, we determined phage concentrations in mouse organs over time after intraperitoneal and intra-articular application of 10

Indexed as

Phage TherapyProsthesis-Related InfectionsStaphylococcal InfectionsStaphylococcus epidermidisStaphylococcus PhagesAnimalsDisease Models, AnimalFemaleMicePeriprosthetic joint infectionPhage manufacturingPhage pharmacokineticsPhage therapyStaphylococci

Identifiers

PMID40456366
PMCPMC12173072

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.