Small extracellular vesicles from human bone marrow mesenchymal stromal cells enhance migration and regulate reparative gene expression in dermal fibroblasts.

Rui Lei, Tamiris Borges da Silva, Zhanfeng Cui, Hua Ye

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rui Lei *Department of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Headington, Oxford, OX3 7DQ, UK.

Tamiris Borges da Silva *Department of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Headington, Oxford, OX3 7DQ, UK.

Zhanfeng CuiDepartment of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Headington, Oxford, OX3 7DQ, UK.

Hua YeDepartment of Engineering Science, Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Headington, Oxford, OX3 7DQ, UK. hua.ye@eng.ox.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studies have shown that mesenchymal stromal cells (MSCs) could secrete a variety of bioactive particles, including small extracellular vesicles (sEVs), that might be a key intermediate to the beneficial paracrine effects of MSC therapy. In this study, we harvested the conditioned medium (CM) from human bone marrow mesenchymal stromal cells (hBM-MSCs) and normal human dermal fibroblasts (NHDFs), fractionated into the sEV fraction and non-small extracellular vesicle (NsEV) fraction, and compared their functions on NHDF migration and proliferation-key processes in wound healing, coupled with transcriptomic analysis through mRNA sequencing to assess gene expression changes in the recipient NHDFs. Our findings show that sEV, NsEV and CM from hBM-MSCs had an overall promotive effect on migration behaviour of NHDFs, but MSC-sEV surpassed the effects of other MSC secretome fractions and their NHDF counterparts (HDF-sEV). Gene ontology analysis revealed enrichment of pathways related to migration, and significant changes in genes within the regulation of proliferation pathway. Our study provides referential significance for the choice of cellular secretome fractions to be used in wound healing studies and insights into the effects of MSC secretome in the migration and proliferation of healthy fibroblasts, besides their effects on gene expression changes.

Indexed as

Cell MovementDermisExtracellular VesiclesFibroblastsGene Expression RegulationMesenchymal Stem CellsCell ProliferationCells, CulturedCulture Media, ConditionedGene Expression ProfilingHumansWound HealingCulture Media, ConditionedExtracellular vesiclesFibroblastsMesenchymal stromal cellsMigrationRNA sequencing

Identifiers

PMID40461530

DOI10.1038/s41598-025-04057-6

PMCPMC12134064

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.