Evidence mapPaperPMID 40461942Full record

ArticleJournal of the American Chemical Society2025

Molecular Design of Unimolecular Tetra-Receptor Agonists.

Tristan C Dinsmore, Jacob E Cortigiano, Siyuan Xiang, Marina V Spenciner, Alexandra R Dobbins, Richard L Zhao, Brett M Waldman, Martin Beinborn, Krishna Kumar

Abstract read
In one paragraph

Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tristan C DinsmoreDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Jacob E CortigianoDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Siyuan XiangDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Marina V SpencinerDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Alexandra R DobbinsDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Richard L ZhaoDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Brett M WaldmanDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Martin BeinbornDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Krishna KumarDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.ORCID 0000-0002-0548-5014

Funding

A Robust Platform for Design and Development of Therapeutic Peptides for Metabolic SyndromeR42DK131842 · VELUM, INC. · 2025 to 2025
$959k
Medical Scientist Training Program at Tufts UniversityT32GM146621 · TUFTS UNIVERSITY BOSTON · 2025 to 2025
$466k
NIA NIH HHS R41 AG061909NIA NIH HHS R42 AG061909NIDDK NIH HHS R41 DK131842NIDDK NIH HHS R42 DK131842NIGMS NIH HHS R41 GM130257NIGMS NIH HHS R41 GM142448NIGMS NIH HHS T32 GM146621
6 · The paper itself

Abstract

Peptide hormone-receptor interactions serve as critical regulators of metabolic homeostasis, a paradigm exemplified by the clinical efficacy of glucagon-like peptide-1 (GLP-1) receptor agonists. Building upon this framework, strategic design has yielded unimolecular dual and triple agonists targeting GLP-1R, glucose-dependent insulinotropic polypeptide receptor (GIPR), and glucagon receptor (GcgR), leveraging the sequence homology within the cognate native ligands of the class B G protein-coupled receptor (GPCR) family. However, the integration of Y2 receptor (Y2R) agonism─engaged by peptide YY (PYY) and belonging to the structurally divergent class A GPCR family─has remained an unaddressed challenge due to the topological and sequence disparities between these receptor classes. Y2R activation plays a pivotal role in appetite suppression, potentiating the metabolic benefits conferred by GLP-1R, GIPR, and GcgR agonism. Here, we report first-in-class, unprecedented tetra-agonists with high potency at GLP-1R, GIPR, GcgR, and Y2R. The chimeric peptides overcome the intrinsic sequence constraints imposed by class A and class B GPCR divergence, demonstrating the feasibility of rationally designed agonism mediated by single agents across receptor families. Lipidation of this template is well tolerated enhancing the promise of therapeutic viability. Furthermore, we show that biased agonism at GLP-1R selectively boosts cyclic AMP (cAMP) signaling while minimizing β-arrestin recruitment, thereby decoupling receptor desensitization from metabolic efficacy. Additionally, we introduce a tunable framework to modulate β-arrestin engagement without compromising cAMP potency, providing insight into the fine-tuning of GPCR-mediated signaling for next-generation peptide therapeutics.

Indexed as

Drug DesignGlucagon-Like Peptide-1 Receptor AgonistsReceptors, Gastrointestinal HormoneReceptors, GlucagonReceptors, Neuropeptide YGlucagon-Like Peptide-1 ReceptorHumansgastric inhibitory polypeptide receptorGlucagon-Like Peptide-1 ReceptorGlucagon-Like Peptide-1 Receptor Agonistsneuropeptide Y2 receptorReceptors, Gastrointestinal HormoneReceptors, GlucagonReceptors, Neuropeptide Y

Identifiers

PMID40461942
PMCPMC12560177

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.