Evidence mapPaperPMID 40464850Full record

ArticleOdontology2026

Waterpipe smoke condensate activates the oncogenes and promotes epithelial-mesenchymal transition to cause oral cancer.

Prathibha Prasad, Gopu Sriram, Balachander Kannan, Mohamed Jaber, Al Moutassem Billah Khair, Abilasha Ramasubramanian, Pratibha Ramani, Vijayashree Priyadharsini Jayaseelan, Paramasivam Arumugam

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Article in Odontology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. DNA methyltransferases expressions in mice tongue exposed to waterpipe smoke.Journal of applied oral science : revista FOB · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Prathibha Prasad *College of Dentistry, Center of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates. p.prasad@ajman.ac.ae.
Gopu SriramFaculty of Dentistry, National University of Singapore, Singapore, Singapore.
Balachander KannanMolecular Biology Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, India.
Mohamed JaberCollege of Dentistry, Center of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates.
Al Moutassem Billah KhairCollege of Dentistry, Center of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates.
Abilasha RamasubramanianDepartment of Oral Pathology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India.
Pratibha RamaniDepartment of Oral Pathology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India.
Vijayashree Priyadharsini JayaseelanClinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India.
Paramasivam Arumugam *Molecular Biology Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, India. paramasivama.sdc@saveetha.com.ORCID http://orcid.org/0000-0001-6117-1178

Funding

Ajman University 2022-IRG-DEN-4
6 · The paper itself

Abstract

Oral squamous cell carcinoma (OSCC) is a significant health challenge, particularly in the Indian subcontinent, where it is the second leading cause of cancer. Smoking, including waterpipe tobacco smoking, is a major risk factor for OSCC and contributes to the onset of malignancy through the absorption of carcinogenic compounds present in tobacco smoke. This study investigated the role of the oncogene Yes-associated protein 1 (YAP1) signaling pathway in OSCC progression, focusing on how exposure to waterpipe smoke condensate (WPSC) influences cellular proliferation, epithelial-mesenchymal transition (EMT), and YAP1 activity. Using immortalized human oral keratinocytes (HOK) and OSCC cell lines, we found that WPSC significantly enhanced cell proliferation, increased colony formation, and elevated YAP1 expression compared to controls. Notably, WPSC treatment resulted in the downregulation of the epithelial marker E-cadherin and the upregulation of mesenchymal markers, indicating a shift towards an invasive phenotype. Further bioinformatic analysis of The Cancer Genome Atlas (TCGA) dataset confirmed that YAP1 is frequently altered in HNSCC, highlighting its role in OSCC carcinogenesis. Our findings establish YAP1 as a central mediator in the response of oral keratinocytes to the oncogenic effects of waterpipe smoking, promoting EMT and enhancing invasive characteristics of OSCC cells. These results underscore the therapeutic potential of targeting the YAP1 signaling pathway in OSCC treatment, particularly in individuals exposed to waterpipe smoke. Future research should elucidate the molecular mechanisms of WPSC-induced YAP1 activation and EMT, paving the way for innovative therapeutic strategies to combat OSCC.

Indexed as

Carcinoma, Squamous CellEpithelial-Mesenchymal TransitionMouth NeoplasmsOncogenesSmokeWater Pipe SmokingAdaptor Proteins, Signal TransducingCell Line, TumorCell ProliferationHumansKeratinocytesSignal TransductionTranscription FactorsYAP-Signaling ProteinsAdaptor Proteins, Signal TransducingSmokeTranscription FactorsYAP1 protein, humanYAP-Signaling ProteinsEpithelial–mesenchymal transitionHealthcareOral squamous cell carcinomaWaterpipe smokingYAP1

Identifiers

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Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.