Evidence map›Paper›PMID 40467700›Full record

ArticleScientific reports2025

Pig models reveal the interplay between fatty acids and cytokines in skeletal muscle.

Fernanda Nery Ciconello, Christopher Keith Tuggle, Julia Dezen Gomes, Bruna Pereira Martins da Silva, Mariah de Castro Durval, Camila Sabino de Oliveira, Lucas Echevarria Nascimento, Luciana Sales Freitas, James Eugene Koltes, Aline Silva Mello Cesar

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Fernanda Nery CiconelloDepartment of Animal Science, "Luiz de Queiroz" College of Agriculture, Av. Pádua Dias, 11, Piracicaba, São Paulo, 13418-900, Brazil.
Christopher Keith TuggleDepartment of Animal Science, Iowa State University, Kildee Hall, 806 Stange Road, Ames, IA, 50011-3150, USA.
Julia Dezen GomesDepartment of Animal Science, "Luiz de Queiroz" College of Agriculture, Av. Pádua Dias, 11, Piracicaba, São Paulo, 13418-900, Brazil.
Bruna Pereira Martins da SilvaDepartment of Animal Science, "Luiz de Queiroz" College of Agriculture, Av. Pádua Dias, 11, Piracicaba, São Paulo, 13418-900, Brazil.
Mariah de Castro DurvalDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering, R. Duque de Caxias, Pirassununga, São Paulo, 225, 13635-900, Brazil.
Camila Sabino de OliveiraDepartment of Animal Science, "Luiz de Queiroz" College of Agriculture, Av. Pádua Dias, 11, Piracicaba, São Paulo, 13418-900, Brazil.
Lucas Echevarria NascimentoDepartment of Animal Science, "Luiz de Queiroz" College of Agriculture, Av. Pádua Dias, 11, Piracicaba, São Paulo, 13418-900, Brazil.
Luciana Sales FreitasDB Genética Company, Av. JK, 2094, Patos de Minas, Minas Gerais, 38706-000, Brazil.
James Eugene KoltesDepartment of Animal Science, Iowa State University, Kildee Hall, 806 Stange Road, Ames, IA, 50011-3150, USA.
Aline Silva Mello CesarDepartment of Food Science and Technology, "Luiz de Queiroz" College of Agriculture, Av. Pádua Dias, 11, Piracicaba, São Paulo, 13418-900, Brazil. alinecesar@usp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fatty acids, particularly those derived from plant and animal oils, play roles in physiological functions and metabolic regulation in pigs. Imbalances in the ratio of polyunsaturated fatty acids (PUFAs) may influence inflammatory responses, including the modulation of pro-inflammatory cytokines. This study aimed to investigate the gene co-expression profiles in the skeletal muscle of pigs fed diets supplemented with 3.0% soybean, canola, or fish oils, correlating these profiles with cytokine abundance and identifying hub genes associated with immune-related pathways using a systems biology approach. Skeletal muscle samples from 27 pigs were subjected to RNA sequencing and weighted gene co-expression network analysis (WGCNA) to construct gene co-expression networks. The concentrations of six cytokines (IL-10, IFN-γ, IL-1β, IL-6, IL-18, and TNF-α) were quantified in muscle tissue using ELISA. Functional enrichment analysis and hub gene identification revealed several key genes involved in immune function and fatty acid metabolism. WGCNA uncovered distinct co-expression modules associated with specific dietary oil treatments. These findings provide new insights into the immunomodulatory effects of soybean, canola, and fish oils, highlighting the relevance of nutrigenomics in understanding gene-diet interactions in pigs.

Indexed as

CytokinesFatty AcidsMuscle, SkeletalAnimal FeedAnimalsDietary SupplementsFish OilsGene Expression ProfilingGene Regulatory NetworksRapeseed OilSwineTranscriptomeCytokinesFatty AcidsFish OilsRapeseed OilELISAGene expressionInflammationLipidsSwineWGCNA

Identifiers

PMID40467700
PMCPMC12137911

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.