Evidence mapPaperPMID 40468753Full record

ArticleIntegrative cancer therapies

A Promising Natural Red Pigment "Prodigiosin" Sensitizes Colon Cancer Cells to Ionizing Radiation, Induces Apoptosis, and Impedes MAPK/TNF-α/NLRP3 Signaling Pathway.

Nermeen M ElBakary, Lobna M Anees, Amal Said Shahat, Noura M Mesalam

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Article in Integrative cancer therapies. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nermeen M ElBakaryRadiation Biology Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.ORCID 0000-0002-0128-3159
Lobna M AneesHealth Radiation Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Amal Said ShahatEgyptian drug Authority (EDA), Formaly national organization for drug control, general division of main medical science, Cairo, Egypt.
Noura M MesalamBiological Application Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colon cancer is one of the most prevalent cancers worldwide and the second leading cause of cancer-related deaths. The goal of the present study was to investigate the role of Prodigiosin (PG) in promoting programed cell death in irradiated Caco-2 colon cancer cells. We examined the extent to which PG disrupts the BCL-2/caspase-3 and PPAR-γ signaling pathways and affects apoptosis in these cancer cells. The inflammatory markers COX2, PGE2, NO, TNFα, and the inflammosome NLRP-3, MAPK in addition to prooxidant/antioxidant balance is also under investigation. Caco-2 cells were irradiated with gamma rays (6 Gy) either with or without PG and the results revealed that, PG established IC50 equivalent to 357.27 μgl/ml in Caco-2 cells. The flowcytometric analysis (Annexin V), BCL-2 and caspase-3 showed that PG induces apoptosis for Caco-2 cells. Furthermore, the PG + gamma irradiated (R) group of Caco-2 cells showed significant down regulation in proliferation and inflammatory cascade induction followed by changes in redox tone (expressed by increase in SOD and GSH activities and decrease in MDA concentration), resulted in reduction of tumor growth. It could be concluded that PG has an anti-proliferative action on Caco-2 cells because of its capability to enhance apoptosis in addition to its capability to enhance response of Caco-2 cells to gamma radiation. Based on our findings in the present study we were able to demonstrate that the oxidative status as well as inflammatory responses are grave for determining the longevity, life span and reactivity of Caco-2 colon cancer cells upon exposure to PG unaccompanied or accompanied by gamma radiation. Prodigiosin might represent a valuable key in contesting development of drug resistance of cancer cells and it could raise the radio-sensitivity of cells when PG delivered in combination with radiation exposures.

Indexed as

ApoptosisColonic NeoplasmsNLR Family, Pyrin Domain-Containing 3 ProteinPigments, BiologicalProdigiosinRadiation-Sensitizing AgentsCaco-2 CellsGamma RaysHumansMAP Kinase Signaling SystemRadiation, IonizingSignal TransductionTumor Necrosis Factor-alphaNLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humanPigments, BiologicalProdigiosinRadiation-Sensitizing AgentsTumor Necrosis Factor-alphaBCL-2Caco-2caspase-3MAPKNLRP-3PPAR-γprodigiosinTNF-αγ-rays

Identifiers

PMID40468753
PMCPMC12144358

What Socratic holds

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LicenceCC BY-NC
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.