Evidence map›Paper›PMID 40469504›Full record

ReviewiMeta2025

The microbiologist's guide to metaproteomics.

Tim Van Den Bossche, Jean Armengaud, Dirk Benndorf, Jose Alfredo Blakeley-Ruiz, Madita Brauer, Kai Cheng, Marybeth Creskey, Daniel Figeys, Lucia Grenga, Timothy J Griffin and 22 more

Abstract readReview
In one paragraph

Review in iMeta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors.

Tim Van Den BosscheDepartment of Biomolecular Medicine, Faculty of Medicine and Health Sciences Ghent University Ghent Belgium.ORCID 0000-0002-5916-2587
Jean ArmengaudDépartement Médicaments Et Technologies Pour La Santé (DMTS) Université Paris-Saclay, CEA, INRAE, SPI, Bagnols-Sur-Cèze France.ORCID 0000-0003-1589-445X
Dirk BenndorfApplied Biosciences and Process Engineering Anhalt University of Applied Sciences Köthen Germany.ORCID 0000-0003-4021-8525
Jose Alfredo Blakeley-RuizDepartment of Plant and Microbial Biology North Carolina State University Raleigh North Carolina USA.ORCID 0000-0001-7638-5849
Madita BrauerMolecular Disease Mechanisms Group, Department of Life Sciences and Medicine University of Luxembourg Esch-sur-Alzette Luxembourg.ORCID 0000-0001-9709-9155
Kai ChengSchool of Pharmaceutical Sciences, Faculty of Medicine University of Ottawa Ottawa Ontario Canada.ORCID 0000-0002-6045-0244
Marybeth CreskeyBiologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch Health Canada Ottawa Ontario Canada.ORCID 0000-0002-5884-1051
Daniel FigeysSchool of Pharmaceutical Sciences, Faculty of Medicine University of Ottawa Ottawa Ontario Canada.ORCID 0000-0002-5373-7546
Lucia GrengaDépartement Médicaments Et Technologies Pour La Santé (DMTS) Université Paris-Saclay, CEA, INRAE, SPI, Bagnols-Sur-Cèze France.ORCID 0000-0001-5560-1717
Timothy J GriffinDepartment of Biochemistry, Molecular Biology, and Biophysics University of Minnesota, Minneapolis Minnesota USA.ORCID 0000-0001-6801-2559
Céline HenryUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute PAPPSO Jouy-en-Josas France.ORCID 0000-0002-2355-1791
Robert L HettichBiosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee USA.ORCID 0000-0001-7708-786X
Tanja HolsteinDepartment of Biomolecular Medicine, Faculty of Medicine and Health Sciences Ghent University Ghent Belgium.ORCID 0000-0002-1552-1453
Pratik D JagtapDepartment of Biochemistry, Molecular Biology, and Biophysics University of Minnesota, Minneapolis Minnesota USA.ORCID 0000-0003-0984-0973
Nico JehmlichDepartment of Molecular Toxicology Helmholtz-Centre for Environmental Research - UFZ GmbH Leipzig Germany.ORCID 0000-0002-5638-6868
Jonghyun KimBiosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee USA.ORCID 0000-0001-9749-6169
Manuel KleinerDepartment of Plant and Microbial Biology North Carolina State University Raleigh North Carolina USA.ORCID 0000-0001-6904-0287
Benoit J KunathDepartment of Plant and Microbial Biology North Carolina State University Raleigh North Carolina USA.ORCID 0000-0002-3356-8562
Xuxa MallietDepartment of Biomolecular Medicine, Faculty of Medicine and Health Sciences Ghent University Ghent Belgium.ORCID 0009-0000-9420-2655
Lennart MartensDepartment of Biomolecular Medicine, Faculty of Medicine and Health Sciences Ghent University Ghent Belgium.ORCID 0000-0003-4277-658X
Subina MehtaDepartment of Biochemistry, Molecular Biology, and Biophysics University of Minnesota, Minneapolis Minnesota USA.ORCID 0000-0001-9818-0537
Bart MesuereDepartment of Mathematics, Statistics and Computer Science, Faculty of Sciences Ghent University Ghent Belgium.ORCID 0000-0003-0610-3441
Zhibin NingSchool of Pharmaceutical Sciences, Faculty of Medicine University of Ottawa Ottawa Ontario Canada.ORCID 0000-0003-2045-7596
Alessandro TancaDepartment of Biomedical Sciences University of Sassari Sassari Italy.ORCID 0000-0002-6066-7048
Sergio UzzauDepartment of Biomedical Sciences University of Sassari Sassari Italy.ORCID 0000-0001-6246-2794
Pieter VerschaffeltDepartment of Biomolecular Medicine, Faculty of Medicine and Health Sciences Ghent University Ghent Belgium.ORCID 0000-0002-6675-1048
Jing WangState Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing China.ORCID 0009-0005-8207-3928
Paul WilmesLuxembourg Centre for Systems Biomedicine University of Luxembourg Esch-sur-Alzette Luxembourg.ORCID 0000-0002-6478-2924
Xu ZhangBiologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch Health Canada Ottawa Ontario Canada.ORCID 0000-0003-2406-9478
Xin ZhangState Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing China.ORCID 0009-0001-9107-1626
Leyuan LiState Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics Beijing China.ORCID 0000-0003-2063-4441
Metaproteomics Initiative

Funding

Metaproteomics to investigate intestinal microbiota-host and -diet interactionsR35GM138362 · NIGMS · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI KLEINER, MANUEL · 2020 to 2024
$1.9M
NIGMS NIH HHS R35 GM138362
6 · The paper itself

Abstract

Metaproteomics is an emerging approach for studying microbiomes, offering the ability to characterize proteins that underpin microbial functionality within diverse ecosystems. As the primary catalytic and structural components of microbiomes, proteins provide unique insights into the active processes and ecological roles of microbial communities. By integrating metaproteomics with other omics disciplines, researchers can gain a comprehensive understanding of microbial ecology, interactions, and functional dynamics. This review, developed by the Metaproteomics Initiative (www.metaproteomics.org), serves as a practical guide for both microbiome and proteomics researchers, presenting key principles, state-of-the-art methodologies, and analytical workflows essential to metaproteomics. Topics covered include experimental design, sample preparation, mass spectrometry techniques, data analysis strategies, and statistical approaches.

Indexed as

bioinformaticsfunctional dynamicsmass spectrometrymetaproteomicsmicrobiome

Identifiers

PMID40469504
PMCPMC12130581

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.