Evidence map›Paper›PMID 40472849›Full record

ArticleCell reports methods2025

Microendoscopy for periodic intravital end-to-end tumor imaging of cancer cells.

Toshiyuki Goto, Masayuki Nakano, Sally Danno, Chie Ueda, Asako Sakaue-Sawano, Atsushi Miyawaki, Anna Wrabel, Ichiro Nakahara, Takahito Nishikata, Akira Mizoguchi and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Toshiyuki GotoRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Hyogo 650-0047, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 650-0047, Japan.
Masayuki NakanoRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan.
Sally DannoRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 650-0047, Japan.
Chie UedaRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 650-0047, Japan.
Asako Sakaue-SawanoRIKEN Center for Brain Science, Wako, Saitama 351-0198, Japan; Biotechnological Optics Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama 351-0198, Japan.
Atsushi MiyawakiRIKEN Center for Brain Science, Wako, Saitama 351-0198, Japan; Biotechnological Optics Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama 351-0198, Japan.
Anna WrabelRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan.
Ichiro NakaharaRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan.
Takahito NishikataFrontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Hyogo 650-0047, Japan.
Akira MizoguchiRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Graduate School of Medicine, Mie University, Tsu, Mie 514-8507, Japan.
Yasuhisa TamuraRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 650-0047, Japan.
Kei MizunoRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 650-0047, Japan.
Yosky KataokaRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 650-0047, Japan; RIKEN-JEOL Collaboration Center, RIKEN Baton Zone Program, Kobe, Hyogo 650-0047, Japan. Electronic address: kataokay@person.kobe-u.ac.jp.
Kazuo FunabikiRIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan; Foundation for Biomedical Research and Innovation, Kobe, Hyogo 650-0047, Japan. Electronic address: funabiki@ent.kuhp.kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The spatiotemporal heterogeneity in intratumor proliferative behavior of cancer cells deeply affects tumor environment characteristics and the efficacy of anticancer treatments. Thus, intravital imaging with unlimited imaging depth and cellular-level resolution is greatly desired. We developed an optical-fiber-bundle-based microendoscope with a genetically encoded fluorescent ubiquitination-based cell-cycle indicator (Fucci) system to achieve the intravital, periodic, and multicolor end-to-end imaging of the proliferative activity of cancer cells at a cellular-level resolution. This technique enabled the periodic visualization of spatiotemporal cellular responses, including cell-cycle arrest and resumption, and nuclear enlargement following the administration of anticancer drugs in living mice. It was suggested that proliferating cell ratio and nuclear enlargement in cancer cells at the surface region of tumor characterized by abundant vascular invasion contribute to aggressive tumor regrowth after chemotherapy. The application of this technique can accelerate innovation in cancer biology and therapeutics.

Indexed as

EndoscopyIntravital MicroscopyNeoplasmsAnimalsCell Line, TumorCell ProliferationHumansMiceMice, Nudecancer environmentchemotherapyCP: cancer biologyCP: imagingin vivo imagingmicroendoscopypolyploid cancer cells

Identifiers

PMID40472849
PMCPMC12272250

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.