Evidence map›Paper›PMID 40474257›Full record

ArticleCell & bioscience2025

Alkylglycerol monooxygenase represses prostanoid biosynthesis in a sex-dependent manner.

Zhigang Rao, Katharina Lackner, Ilaria Dorigatti, Natascha Brigo, Denise Kummer, Minh Bui Hoang, Christa Pfeifhofer-Obermair, Günter Weiss, Ernst R Werner, Andreas Koeberle and 1 more

Abstract read
In one paragraph

Article in Cell & bioscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhigang Rao *Michael Popp Institute and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0000-0002-9918-9100
Katharina Lackner *Institute of Human Genetics, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0000-0003-3260-9139
Ilaria DorigattiInstitute of Molecular Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0009-0000-1775-6224
Natascha BrigoDepartment of Internal Medicine II, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0000-0002-4002-7852
Denise KummerInstitute of Molecular Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0009-0007-6732-0972
Minh Bui HoangMichael Popp Institute and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0009-0009-4454-2956
Christa Pfeifhofer-ObermairDepartment of Internal Medicine II, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0000-0002-9942-6540
Günter WeissDepartment of Internal Medicine II, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0000-0003-0709-2158
Ernst R WernerInstitute of Molecular Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.ORCID http://orcid.org/0000-0003-1948-3391
Andreas Koeberle *Michael Popp Institute and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria. andreas.koeberle@uni-graz.at.ORCID http://orcid.org/0000-0001-6269-5088
Katrin Watschinger *Institute of Molecular Biochemistry, Biocenter, Medical University of Innsbruck, Innsbruck, Austria. katrin.watschinger@i-med.ac.at.ORCID http://orcid.org/0000-0002-1122-8444

Funding

Austrian Science Fund 10.55776/FG15Austrian Science Fund 10.55776/P34723Phospholipid Research Center AKO-2019-070/2-1Phospholipid Research Center AKO-2O22-100/2-2
6 · The paper itself

Abstract

backgroundEther lipids are important constituents of biological membranes and harbor fatty alcohols attached via ether linkages to the sn-1 position of the glycerol backbone. Depending on the nature of the ether bond, they are subdivided into 1-O-alkyl (plasmanyl) and 1-O-alk-1'-enyl (plasmenyl) subclasses. They often contain polyunsaturated fatty acids at the sn-2 position, implicating them in cellular signaling and inflammatory processes including lipid mediator biosynthesis. Lipid mediators are produced by immune and non-immune cells, have diverse homeostatic and immunoregulatory functions and, together with other factors, orchestrate the initiation and resolution of inflammation. To date, alkylglycerol monooxygenase is the only known enzyme capable of cleaving alkylglycerols, one of two ether lipid subclasses. However, the exact role of alkylglycerol monooxygenase and that of its substrates in lipid mediator biosynthesis remains unclear.

resultsUsing a knockout mouse model, we demonstrate a sex- and cell type-dependent role of alkylglycerol monooxygenase in limiting prostanoid formation without affecting polyunsaturated fatty acid release, as revealed by metabololipidomics profiling of lipid mediators using ultra-performance liquid chromatography‒tandem mass spectrometry. This female-specific effect is driven by the suppression of prostaglandin G/H synthase 2 transcription, as deficiency in alkylglycerol monooxygenase significantly elevated prostaglandin G/H synthase 2 gene expression in female bone marrow-derived macrophages of the M1 phenotype. Furthermore, this regulatory role of alkylglycerol monooxygenase extends to visceral white adipose tissue, where elevated prostaglandin G/H synthase 2 expression and enhanced prostaglandin E

conclusionOur results expand the immunomodulatory functions of ether lipid metabolism and highlight the sex- and cell type-dependent role of alkylglycerol monooxygenase in controlling lipid mediator production and maintaining tissue homeostasis.

Indexed as

AGMOEther lipidLipid mediatorLipidomicsPolyunsaturated fatty acid

Identifiers

PMID40474257
PMCPMC12143016

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.