Evidence map›Paper›PMID 40478496›Full record

ArticleJournal of plant research2025

Isolation and functional identification of Jasmonte resistant 1, a Jasmonic acid isoleucine-conjugating enzyme in Catharanthus roseus.

Aikun Pan, Haiqiao Gao, Ying Lin, Bingrun Yang, Chunhao Chang, Zhiwen Liu, Yanyan Wang, Zhaoxia Jin, Fang Yu

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In one paragraph

Article in Journal of plant research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Aikun PanSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Haiqiao GaoSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Ying LinSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Bingrun YangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Chunhao ChangCollege of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China.
Zhiwen LiuSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Yanyan WangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China. wang_yy@dlpu.edu.cn.
Zhaoxia JinSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China. jinzx2018@163.com.
Fang YuSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China. fyu0506@gmail.com.

Funding

Liaoning Provincial Department of Human Resources and Social Security JYTMS20230433Major Scientific and Technological Special Project of Guizhou Province 2019FY100500National Natural Science Foundation of China 31970324National Natural Science Foundation of China 42177112
6 · The paper itself

Abstract

Jasmonate resistant 1 (JAR1), which encodes a jasmonate-amino synthase, mediates the biosynthesis of jasmonoyl-isoleucine (JA-Ile), the bioactive form of jasmonate involved in JA signal transduction pathway. Although JAR1 has been characterized in several plant species, its regulatory function in mediating JA signaling to modulate the biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus, a medicinal and ornamental plant, have not been previously investigated. In this study, we have identified and cloned CrJAR1, a member of the Gretchen Hagen3 (GH3) gene family, from C. roseus and confirmed its enzymatic activity in JA-Ile formation via heterologous expression in E. coli. Functional analyses using transient overexpression and virus-induced gene silencing (VIGS) in C. roseus leaves revealed that CrJAR1 positively regulates the accumulation of catharanthine and vindoline. Moreover, we demonstrated that CrCOI1, the jasmonate receptor, functions synergistically with CrJAR1 in modulating TIA biosynthesis. Co-silencing of CrCOI1 and CrJAR1 resulted in a lower sensitivity to exogenous JA treatment compared to silencing of CrJAR1 alone, indicating distinct yet complementary roles in jasmonate-mediated metabolic regulation. These results advance our understanding the function of CrJAR1 and offer new strategies for engineering specialized metabolite biosynthesis in plants.

Indexed as

CatharanthusCyclopentanesIsoleucineOxylipinsPlant ProteinsGene Expression Regulation, PlantGene SilencingPlant Growth RegulatorsSecologanin Tryptamine AlkaloidsVinblastineVinca AlkaloidscatharanthineCyclopentanesIsoleucinejasmonic acidjasmonoyl-isoleucineOxylipinsPlant Growth RegulatorsPlant ProteinsSecologanin Tryptamine AlkaloidsVinblastineVinca AlkaloidsvindolineBiosynthesis of catharanthine and vindolineCatharanthus roseusJA signalingJasmonte resistant 1 (JAR1)

Identifiers

PMID40478496

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.