Evidence mapPaperPMID 40481542Full record

ArticleEuropean journal of medical research2025

CBL-mediated AQP1 ubiquitination aggravates kidney-yang deficiency syndrome by promoting lipid metabolism dysregulation.

Yifei Wang, Jun Yuan, Wenfang He, Nan Yang, Lanjun Fu, Juan Jin

Abstract read
In one paragraph

Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yifei Wang *Department of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310000, China.
Jun Yuan *Department of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310000, China.
Wenfang HeDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310000, China.
Nan YangDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310000, China.
Lanjun FuDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310000, China. fulanjun2013@163.com.
Juan JinDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310000, China. 20233004@zcmu.edu.cn.

Funding

Science Research Fund Project of Zhejiang Chinese Medical University 2020ZG37
6 · The paper itself

Abstract

objectiveKidney-Yang Deficiency Syndrome (KYDS) is associated with lipid metabolism dysregulation. This study aimed to investigate the effect of casitas B-lineage lymphoma (CBL)-mediated aquaporin 1 (AQP1) ubiquitination on KYDS.

methodsThe KYDS rat model was induced via intraperitoneal injection of hydrocortisone. Body weight, body temperature, sperm motility, serum hormone levels, organ indices, and histopathological changes of rats were evaluated. AQP1 was knocked down to detect the effect of AQP1 on lipid metabolism in KYDS rats. Immunofluorescence and immunoprecipitation were used to verify the relationship between CBL and AQP1, and CBL knockdown KYDS rats were constructed to test the effect of CBL on AQP1 ubiquitination.

resultsAQP1 expression was downregulated in KYDS rats. Knockdown of the AQP1 gene in KYDS rats resulted in decreased body weight, body temperature, sperm motility, and testicular index, along with increased renal index. It also resulted in changes of serum hormone levels and exacerbated pathologic changes. Additionally, AQP1 knockdown further suppressed lipid accumulation in KYDS rats, as evidenced by reductions in lipid droplets and the expression of lipid synthesis proteins. Intriguingly, elevated expression of CBL was observed in KYDS, and its knockdown inhibited the ubiquitin-mediated degradation of AQP1. The inhibition of CBL in KYDS improved lipid metabolism dysregulation, thereby ameliorating KYDS.

conclusionCBL-mediated AQP1 ubiquitination aggravates KYDS by promoting lipid metabolism dysregulation, offering promising insights for targeted therapeutic interventions.

Indexed as

Aquaporin 1Kidney DiseasesLipid MetabolismYang DeficiencyAnimalsKidneyMaleRatsRats, Sprague-DawleyUbiquitinationAqp1 protein, ratAquaporin 1Aquaporin 1Casitas B-lineage lymphomaKidney-Yang Deficiency SyndromeLipid metabolismUbiquitination

Identifiers

PMID40481542
PMCPMC12142927

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.