ArticlePlant biotechnology journal2025
Cell death-inducing DNA fragmentation factor alpha (DFFA)-like effectors (CIDEs) improve lipid droplet (LD) formation and oil accumulation in plant tissues.
Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- SNP-based association analysis of key genes involved in triglyceride biosynthesis reveals genetic regulation of oil content and fatty acid composition in tree peony seeds.BMC plant biology · 2026Article
- TargetGAN: A generative AI framework for the design of plant core promoters with targeted activity.Plant communications · 2026Article
- BnaC03.SEIPIN1 improves seed oil content by increasing the number of lipid droplets in Brassica napus.BMC plant biology · 2026Article
- Plant Lipid Droplets and Derived Lipidic Nano-Assemblies: Structure, Biogenesis and Pharmaceutical Applications.Plant biotechnology journal · 2026Review
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Authors and funding
6 authors.
Funding
Abstract
The cell death-inducing DNA fragmentation factor alpha (DFFA)-like effectors (CIDEs), including CIDEA, CIDEB and CIDEC, regulate the number and size of lipid droplets (LDs) in vertebrates. However, no homologue of CIDE has been reported in plants. Here, we expressed the coding sequences of CIDEA, CIDEB and CIDEC from Mus musculus in tobacco leaves and Brassica napus seeds. The results showed that the size and number of LDs in transiently transformed tobacco leaves were 2.8-3.1 times larger and 24-25 fold more numerous compared to mock-infiltrated leaves, respectively. The total fatty acid (FA) content in CIDE-expressing leaves was increased by more than 50%, and the triacylglycerol (TAG) content was increased by 2-3 fold compared to that of mock-infiltrated leaves. In B. napus seeds, the number of LDs was significantly decreased while the size of LDs was greatly enlarged in CIDE-expressing lines, and the TAG was increased by 10.5%-15.3%. However, the contents of phosphatidylcholine (PC), phosphatidic acid (PA), and phosphatidylserine (PS) were significantly reduced. Almost all metabolites in the glycolytic pathway and some metabolites in the tricarboxylic acid (TCA) cycle were higher in CIDE-expressing seeds compared with WT, and the transcript levels of the related genes were significantly higher than those in WT. The results suggest that the expression of mammalian CIDEs can alter the morphology of LDs and increase oil content in different plant tissues. By enhancing the seed oil content in B. napus, our results offer a new biotechnology strategy to improve oil yield.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.