Evidence map›Paper›PMID 40492939›Full record

ArticleAngewandte Chemie (International ed. in English)2025

A Combined Aptamer Pulldown-DNAzyme Cleavage Assay for Intact Methicillin Resistant Staphylococcus aureus Cells.

Monsur Ali, Abigail F Almeida, Dawn White, Alfredo Capretta, John D Brennan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Advances in biosensor technologies for the detection of antimicrobial resistance inFrontiers in cellular and infection microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Monsur AliBiointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
Abigail F AlmeidaBiointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
Dawn WhiteBiointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
Alfredo CaprettaBiointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
John D BrennanBiointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.ORCID 0000-0003-3461-9824

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN184073-18The Ontario Ministry for Research and Innovation RE07-045
6 · The paper itself

Abstract

Accurate and convenient detection of methicillin resistant Staphylococcus aureus (MRSA) plays a vital role in determining appropriate antibiotic interventions. Herein, we report the first example combining a DNA aptamer and an RNA-cleaving DNAzyme (RCD) that bind different protein markers for selective preconcentration and detection of MRSA. An aptamer for the penicillin binding protein 2a (PBP2a) was generated by in vitro selection and was coupled to agarose beads to allow rapid pull down of intact MRSA cells from complex samples. A previously reported RCD for Staphylococcus aureus (SA) was then used to detect a second proteinaceous marker within the lysed cells based on a protein-activated cleavage reaction that was linked to both fluorescence and lateral flow assays, allowing for selective detection of MRSA over methicillin sensitive SA. An optimized LFD assay could detect ∼10

Indexed as

Aptamers, NucleotideDNA, CatalyticMethicillin-Resistant Staphylococcus aureusHumansPenicillin-Binding ProteinsAptamers, NucleotideDNA, CatalyticPenicillin-Binding ProteinsAptamerBioassayDNAzymeIn vitro diagnosticMethicillin resistant staphylococcus aureus

Identifiers

PMID40492939
PMCPMC12338438

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.