Methods of a Reproducible Mouse Model of Clostridioides difficile Infection to Investigate Novel Bacterial-Based Therapies.

Katya Douchant, Jill Greenlaw, Prameet M Sheth

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Katya DouchantDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.

Jill GreenlawGastrointestinal Disease Research Unit (GIDRU), Queen's University, Kingston, ON, Canada.

Prameet M ShethDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. Prameet.Sheth@queensu.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mice are widely used in science and have played an instrumental role in understanding immune mechanisms, testing cancer therapies, and serving as model systems to better understand physiological processess. Mouse models have also been widely used in infectious disease research including in Clostridioides difficile infection (CDI). Mice display similar disease characteristics to human CDI, including susceptibility to disease, clinical symptoms, and pathology, and thus are an ideal in vivo model to investigate treatments. Here we describe a reliable, reproducible model of CDI that allows researchers to investigate the use of prophylactic oral treatments for CDI in mice. Using this model, we report on treatment efficacy using several parameters as a measure of CDI, including survival, bodyweight, colon length, and colonic histology.

Indexed as

Anti-Bacterial AgentsClostridioides difficileClostridium InfectionsDisease Models, AnimalAnimalsColonHumansMiceAnti-Bacterial AgentsClostridioides difficileHistologyInfectionMouse modelToxin

Identifiers

PMID40498310

DOI10.1503/cmaj.180013

What Socratic holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.