Evidence map›Paper›PMID 40499536›Full record

ArticleNeuron2025

The cell-surface shared proteome of astrocytes and neurons and the molecular foundations of their multicellular interactions.

Ling Wu, Vijaya Pandey, Vanessa H Casha, Zhe Qu, Yasaman Jami-Alahmadi, Viviana Gradinaru, James A Wohlschlegel, Baljit S Khakh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. IGF2BP3 remodels the microRNA targeting landscape in MLL-AF4 leukemia.bioRxiv : the preprint server for biology · 2025
    Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ling WuDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Vijaya PandeyDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Vanessa H CashaDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Zhe QuDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Yasaman Jami-AlahmadiDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Viviana GradinaruDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
James A WohlschlegelDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Baljit S KhakhDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA. Electronic address: bkhakh@mednet.ucla.edu.

Funding

Fundamental astrocyte biology in intact neural circuitsR35NS111583 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Baljit Khakh · 2019 to 2026
$7.9M
Astrocyte and neuron brain-region and compartment-specific proteome dynamics in aging and Alzheimer’s diseaseR01AG075955 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT, VOSSEL, KEITH ALAN · 2021 to 2025
$5.2M
New tools to target, identify and characterize astrocytes in the adult nervous systemR01DA047444 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GRADINARU, VIVIANA, KHAKH, BALJIT · 2018 to 2020
$3.3M
Functions and mechanisms of a subpopulation of striatal astrocytesR01MH134926 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Baljit Khakh · 2024 to 2026
$1.2M
NIA NIH HHS R01 AG075955NIDA NIH HHS R01 DA047444NIMH NIH HHS R01 MH134926NINDS NIH HHS R35 NS111583
6 · The paper itself

Abstract

Neurons and astrocytes are predominant brain cells that extensively interact, but the molecular basis of their interactions remains largely unexplored. We identified and mapped striatal astrocytic and neuronal cell-surface proteins (CSPs) and found that many were shared, representing the cell-surface shared proteome of astrocytes and neurons (CS SPAN) bridging striatal astrocyte-neuron interaction sites. CS SPAN was replete with extracellular matrix proteins, cell adhesion molecules, transporters, ion channels, and G protein-coupled receptors. By mapping the cellular origins of astrocytic CSPs, we identified astrocytic interactions with diverse parenchymal cells. Broadly concordant with human data, in a mouse model of Huntington's disease (HD), pathophysiology and its genetic attenuation were accompanied by altered and restored CS SPAN and CSPs, respectively. CS SPAN also included molecules dysregulated in diverse brain disorders. Our study reveals the astrocyte-neuron interface in molecular terms and provides a mechanistic foundation for exploring its physiological roles and contributions to brain diseases.

Indexed as

AstrocytesCell CommunicationMembrane ProteinsNeuronsProteomeAnimalsCorpus StriatumDisease Models, AnimalHumansHuntington DiseaseMiceMice, TransgenicMembrane ProteinsProteomeastrocytecell-surface proteinsextracellular matrixgliahorseradish peroxidaseHuntington's diseaseneuronproteomicsstriatum

Identifiers

PMID40499536
PMCPMC12354340

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.