Evidence map›Paper›PMID 40501037›Full record

ArticleJournal of the American Chemical Society2025

Bis(trifluoromethyl)carborhodamines: Highly Fluorogenic, Far-Red to Near-Infrared Dyes for Live Cell Fluorescence Microscopy, Activity-Based Sensing, and Single-Molecule Microscopy.

Nels C Gerstner, Jack T McCann, Julia G Martin, Katharine M Henn, Kathrin Riske, Sathvik Anantakrishnan, Thomas G W Graham, Xavier Darzacq, Evan W Miller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nels C GerstnerDepartment of Chemistry, University of California, Berkeley, Berkeley , California94720-1460, United States.
Jack T McCannDepartment of Chemistry, University of California, Berkeley, Berkeley , California94720-1460, United States.ORCID 0000-0002-8812-829X
Julia G MartinDepartment of Chemistry, University of California, Berkeley, Berkeley , California94720-1460, United States.
Katharine M HennHelen Wills Neuroscience Institute, University of California, Berkeley, Berkeley , California94720-1460, United States.
Kathrin RiskeDepartment of Chemistry, University of California, Berkeley, Berkeley , California94720-1460, United States.
Sathvik AnantakrishnanBiophysics Graduate Program, University of California, Berkeley, Berkeley , California94720-1460, United States.
Thomas G W GrahamMolecular & Cell Biology, University of California, Berkeley, Berkeley , California94720-1460, United States.
Xavier DarzacqMolecular & Cell Biology, University of California, Berkeley, Berkeley , California94720-1460, United States.
Evan W MillerDepartment of Chemistry, University of California, Berkeley, Berkeley , California94720-1460, United States.ORCID 0000-0002-6556-7679

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Chemical Biology Training ProgramT32GM152983 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MATTHEW B FRANCIS, Evan Walker Miller · 2024 to 2026
$1.5M
Chemical Approaches to Visualize and Quantify Cellular PhysiologyR35GM153237 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Evan Walker Miller · 2024 to 2026
$1.3M
Acquisition of a Cryoprobe and Related Accessories for a 600 MHz NMR Spectrometer to Support Health Research Activities at the University of California, BerkeleyS10OD024998 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI HARTWIG, JOHN F · 2018 to 2018
$265k
Design, synthesis and application of long wavelength fluorophores for bioimagingF32GM139263 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI GERSTNER, NELS · 2021 to 2022
$134k
NIGMS NIH HHS F32 GM139263NIGMS NIH HHS R35 GM153237NIGMS NIH HHS T32 GM066698NIGMS NIH HHS T32 GM152983NIH HHS S10 OD024998
6 · The paper itself

Abstract

Synthetic fluorophores built on a classic rhodamine scaffold are essential for modern microscopy. An attractive feature of synthetic fluorophores is their potential to access long-wavelength excitation and emission profiles (>650 nm) that are difficult to achieve through genetically encoded methods like fluorescent proteins. Here, we present a new strategy to achieve excitation and emission above 650 nm: bis(trifluoromethyl)carborhodamine dyes, or BF dyes. In BF dyes, the geminal methyl groups of carborhodamines are replaced with trifluoromethyl (CF

Indexed as

Fluorescent DyesRhodaminesSingle Molecule ImagingHeLa CellsHumansMicroscopy, FluorescenceMolecular StructureFluorescent DyesRhodamines

Identifiers

PMID40501037
PMCPMC12234253

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.