Evidence map›Paper›PMID 40501083›Full record

ArticleLiver international : official journal of the International Association for the Study of the Liver2025

Targeting the Liver Serine Protease TMPRSS6 Ameliorates Steatosis and Attenuates Fibrosis in Experimental MASLD.

Mariateresa Pettinato, Valeria Furiosi, Rossana Carleo, Letizia Bavuso Volpe, Shuling Guo, Valeria Mannella, Giovanna Musco, Enrica Gilberti, Giuseppe De Palma, Giorgia Federico and 9 more

Erratum issuedAbstract read
In one paragraph

Article in Liver international : official journal of the International Association for the Study of the Liver, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Mariateresa PettinatoRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.ORCID 0000-0002-3259-4865
Valeria FuriosiRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Rossana CarleoRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Letizia Bavuso VolpeRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Shuling GuoIonis Pharmaceuticals, Inc., Carlsbad, California, USA.
Valeria MannellaCOSR-Centre for Omics Sciences, IRCCS Ospedale San Raffaele, Milan, Italy.
Giovanna MuscoBiomolecular NMR Laboratory, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Enrica GilbertiDepartment of Occupational Health, Hygiene, Toxicology and Prevention, ASST Spedali Civili di Brescia, Brescia, Italy.
Giuseppe De PalmaDepartment of Occupational Health, Hygiene, Toxicology and Prevention, ASST Spedali Civili di Brescia, Brescia, Italy.
Giorgia FedericoDepartment of Molecular Medicine and Medical Biotechnology (DMMBM), Università Degli Studi di Napoli Federico II, Naples, Italy.ORCID 0000-0001-9420-6699
Francesca CarlomagnoDepartment of Molecular Medicine and Medical Biotechnology (DMMBM), Università Degli Studi di Napoli Federico II, Naples, Italy.
Alessandro CherubiniPrecision Medicine-Biological Resource Center and Department of Transfusion Medicine, Fondazione IRCCS ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.ORCID 0000-0003-4756-2046
Serena PelusiPrecision Medicine-Biological Resource Center and Department of Transfusion Medicine, Fondazione IRCCS ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Anxhela DanoRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Antonella NaiRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Luca ValentiPrecision Medicine-Biological Resource Center and Department of Transfusion Medicine, Fondazione IRCCS ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.ORCID 0000-0001-8909-0345
Sandro AltamuraDepartment of Pediatric Oncology, Hematology and Immunology, University Hospital Heidelberg, Heidelberg, Germany.
Alessia PaganiRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.
Laura SilvestriRegulation of Iron Metabolism Unit, Division of Genetics and Cell Biology, IRCCS Ospedale San Raffaele, Milan, Italy.ORCID 0000-0002-8726-9808

Funding

DFG (FerrOs-FOR5146)Fondazione Cariplo (Grant no. 2022-0583)FRRB-Early Career Award (1749055)Gilead (IN-IT-989-5790)Italian Ministry of Health, Ricerca Finalizzata PNRR 2022 (PNRR-MAD-2022-12375656)Italian Ministry of Health (Ricerca Finalizzata RF-2021-12373889)Italian ministry of Research (MUR) PNRR-M4-C2 "National Center for Gene Therapy and Drugs based on RNA Technology" CN3, Spoke 4 "ASSET: A sex-specific approach to NAFLD targeting"Italian Ministry of Research (PRIN2022XJNWRM)Telethon grant GMR23T1037The European Union, H2020-ICT-2018-20/H2020-ICT-2020-2 programme "Photonics" (101016726-REVEAL)The European Union, HORIZON-MISS-2021-CANCER-02-03 programme "Genial" (101096312)
6 · The paper itself

Abstract

BACKGROUND AND

aimsMetabolic dysfunction-associated steatotic liver disease (MASLD) is the most common cause of liver disease and a leading contributor to liver-related morbidity and mortality. Currently, no pharmacological approach has demonstrated consistent and long-lasting benefits across all patients. Therefore, identifying new therapeutic targets remains an urgent clinical need. The hepatic serine protease matriptase-2, encoded by TMPRSS6, inhibits the BMP-SMAD pathway. Interestingly, reduced BMP-SMAD signalling in the liver is frequently associated with altered lipid metabolism in patients. Conversely, inactivation of Tmprss6 has been linked to reduced high-fat diet-induced obesity. Based on these findings, we hypothesize that TMPRSS6 represents a novel and promising target for the treatment of MASLD.

methodsHepatic TMPRSS6 expression was analysed in obese patients with or without MASLD. Adult male mice were fed a MASLD-MASH diet, and once hepatosteatosis was established, they were treated with antisense oligonucleotides targeting Tmprss6 while continuing the dietary regimen for an additional 6 weeks.

resultsThe expression of the BMP-SMAD inhibitor TMPRSS6 was increased in people with MASLD and negatively correlated with PPARα signaling, a key regulator of hepatic lipid metabolism. In experimental MASLD, downregulation of hepatocytic Tmprss6 using GalNAc-ASO significantly reduced steatohepatitis and fibrosis and attenuated MASLD-MASH-associated ferroptosis by reshaping hepatic transcription factor activity towards PPARα and SMAD4/SMAD5-driven signalling. Consistently, enhanced BMP-SMAD signalling increased PPARα activity in vivo.

conclusionsOur findings reveal a novel functional crosstalk between TMPRSS6 and PPARα. Pharmacological downregulation of Tmprss6 in experimental MASLD mitigates hepatosteatosis, inflammation and fibrosis by enhancing PPARα signalling and attenuating ferroptosis.

Indexed as

Fatty LiverLiverLiver CirrhosisMembrane ProteinsSerine EndopeptidasesAdultAnimalsDiet, High-FatDisease Models, AnimalFemaleHumansMaleMiceMice, Inbred C57BLMiddle AgedObesitymatriptase 2Membrane ProteinsOligonucleotides, AntisenseSerine EndopeptidasesTMPRSS6 protein, humanantisense oligonucleotidesBMP‐SMADferroptosisMASLDPPARαTMPRSS6

Identifiers

PMID40501083
PMCPMC12159589

What Socratic holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.