Evidence map›Paper›PMID 40501753›Full record

ArticlebioRxiv : the preprint server for biology2025

A Comprehensive Workflow for Imaging Live Insulin Secretion Events and Granules in Intact Islets.

Margret A Fye, Rahul Sharma, Phyu Sin M Myat, Pi'ilani Regan, Hudson McKinney, Guoqiang Gu, Irina Kaverina

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Margret A FyeDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.ORCID 0000-0003-4685-2180
Rahul SharmaDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
Phyu Sin M MyatDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
Pi'ilani ReganDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
Hudson McKinneyDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
Guoqiang GuDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.ORCID 0000-0003-0772-9139
Irina KaverinaDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.

Funding

MULTIDISCIPLINARY TRAINING IN MOLECULAR ENDOCRINOLOGYT32DK007563 · NIDDK · VANDERBILT UNIVERSITY · PI Richard M O'Brien · 1988 to 2026
$15.9M
Microtubule Regulation of Pancreatic Beta Cell Function and DiabetesR01DK106228 · NIDDK · VANDERBILT UNIVERSITY · PI Guoqiang Gu, William Holmes · 2016 to 2026
$6.4M
The Mechanisms of Islet Cell Specification and DifferentiationR01DK065949 · NIDDK · VANDERBILT UNIVERSITY · PI GU, GUOQIANG · 2004 to 2017
$4.4M
Dynamic architecture of microtubule networksR35GM127098 · NIGMS · VANDERBILT UNIVERSITY · PI Irina Kaverina · 2018 to 2026
$4.1M
The DNA methylome-based regulation of functional beta-cell massR01DK125696 · NIDDK · VANDERBILT UNIVERSITY · PI Guoqiang Gu · 2020 to 2026
$3.4M
Cytoskeleton-mediated regulation of insulin secretion hot spots in pancreatic beta cellsF31DK136344 · NIDDK · VANDERBILT UNIVERSITY · PI FYE, MARGRET A · 2023 to 2025
$74k
NIDDK NIH HHS F31 DK136344NIDDK NIH HHS R01 DK065949NIDDK NIH HHS R01 DK106228NIDDK NIH HHS R01 DK125696NIDDK NIH HHS T32 DK007563NIGMS NIH HHS R35 GM127098
6 · The paper itself

Abstract

Accurate detection of insulin secretion from pancreatic beta cells is crucial for understanding normal physiological insulin secretion and its pathophysiological counterpart in diabetic states. Traditional methods using fluorescently labeled insulin granules or dye labeling often struggle to distinguish secretion from insulin granule dynamics. We present an optimized protocol using the cell-impermeable Zn

Identifiers

PMID40501753
PMCPMC12154816

What Socratic holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.