Evidence map›Paper›PMID 40503884›Full record

ArticleApplied and environmental microbiology2025

A recombineering-based platform for high-throughput genomic editing in

Zeyu Liang, Chaoyong Huang, Yitian Li, Chao Yang, Ning Wang, Xiaoyan Ma, Yi-Xin Huo

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zeyu Liang *Center for Future Foods, Muyuan Laboratory, Zhengzhou, Henan Province, China.ORCID 0000-0002-4887-2874
Chaoyong Huang *Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing, China.
Yitian LiKey Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing, China.
Chao YangKey Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing, China.
Ning WangKey Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing, China.ORCID 0000-0001-5406-4286
Xiaoyan MaCenter for Future Foods, Muyuan Laboratory, Zhengzhou, Henan Province, China.ORCID 0000-0002-3558-4486
Yi-Xin HuoCenter for Future Foods, Muyuan Laboratory, Zhengzhou, Henan Province, China.ORCID 0000-0003-4644-999X

Funding

Natural Science Foundation of Hebei Province C2024105020The foundation of Muyuan laboratory 11116022401
6 · The paper itself

Abstract

Functional analysis of bacterial genes or genomic fragments IMPORTANCE: With the increasing demand in the microbiology field and the expansion of its application scope, the urgency for genome editing techniques that are not only efficient and versatile but also capable of high-throughput processing and even automation has become increasingly critical. In this study, we enhanced the efficiency of recombination engineering by incorporating modifications and integrated it with the CRISPR system to develop an advanced gene editing method. This method allows for various gene editing events such as insertion, replacement, and long fragment knockout without the need for plasmid construction. It not only demonstrated high efficiency in common

Indexed as

Escherichia coliGene EditingGenome, BacterialCRISPR-Cas SystemsRecombination, Geneticbacterial genomicsgenomic editinghigh-throughput engineeringrecombineering

Identifiers

PMID40503884
PMCPMC12285239

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.