Evidence mapPaperPMID 40505639Full record

ArticleKidney & blood pressure research2025

Construction of a PANoptosis-Related Gene Signature for Diabetic Nephropathy.

Li Geng, Yingying Liu, Yunwei Sun, Yan Chen

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Article in Kidney & blood pressure research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Li GengDepartment of Vascular Surgery, The second hospital of Jilin University, Changchun, China.
Yingying LiuDepartment of Endocrinology, The second hospital of Jilin University, Changchun, China.
Yunwei SunDepartment of Endocrinology, The second hospital of Jilin University, Changchun, China.
Yan ChenDepartment of Endocrinology, The second hospital of Jilin University, Changchun, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionDiabetic nephropathy (DN) is a serious complication of diabetes. In this study, we aimed to develop a diagnostic model for DN based on PANoptosis-related genes.

methodsPANoptosis-related differentially expressed gene (DEGs) associated with DN were identified in the GSE96804 and GSE142025 datasets. Pairwise correlations among these genes were assessed via Pearson correlation analysis. Immune cell abundance in DN patients versus controls was compared in GSE96804. Feature genes for DN prediction were selected with machine learning, and a diagnostic model was constructed using LASSO regression. High-risk and low-risk groups were established based on risk scores, with GSEA used to explore enriched biological processes and pathways. The association between risk scores and immune cell infiltration was examined using CIBERSORT. Potential therapeutic drugs were investigated via the DGIdb database.

resultsSix PANoptosis-related DEGs were found. Immune cell analysis showed significant differences in dendritic cells, macrophages, mast cells, and neutrophils between DN patients and controls. A diagnostic model using three genes (PDK4, YWHAH, PRKX) achieved high accuracy (area under the curve = 0.8-1.0) across datasets, with a reliable nomogram for DN prediction. Risk stratification linked higher risk scores to distinct immune infiltration patterns and enriched cellular transport and metabolic pathways in high-risk DN patients. Protein-protein interaction network and correlation analyses revealed complex gene interactions. Potential therapeutic targets (PRKX, PDK4) and drugs were identified, and quantitative PCR validated YWHAH upregulation in patient plasma samples.

conclusionThe integration of PANoptosis-related genes PDK4, YWHAH, and PRKX offers a promising diagnostic model for DN, with YWHAH potentially involved in the pathological progression of DN.

Indexed as

Diabetic NephropathiesGene Expression ProfilingHumansPyruvate Dehydrogenase Acetyl-Transferring KinasePDK4 protein, humanPyruvate Dehydrogenase Acetyl-Transferring KinaseBiomarkerCluster analysisDiabetic nephropathyMacrophagesRisk assessment

Identifiers

PMID40505639
PMCPMC12263135

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