Evidence mapPaperPMID 40514348Full record

ArticleCell proliferation2025

Mechanism of EC-EXOs-Derived THBS3 Targeting CD47 to Regulate BMSCs Differentiation to Ameliorate Bone Loss.

Jiaojiao Wang, Zhaokai Zhou, Wenjie Chen, Yun Chen, Qiyue Zheng, Yajun Chen, Zhengxiao Ouyang, Ran Xu, Qiong Lu

Abstract read
In one paragraph

Article in Cell proliferation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiaojiao WangDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Zhaokai ZhouDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Wenjie ChenDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Yun ChenDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Qiyue ZhengDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Yajun ChenDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Zhengxiao OuyangDepartment of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China.ORCID https://orcid.org/0000-0002-8997-0446
Ran XuDepartment of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, People's Republic of China.
Qiong LuDepartment of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.ORCID https://orcid.org/0000-0002-0126-4978

Funding

Hunan Innovative Province Construction Special Project 2021ZK4025Hunan Provincial Department of Finance Grant 2020-83Hunan Provincial Department of Finance Grant 2021-139Hunan Provincial Department of Finance Grant 2022-151Hunan Provincial Department of Finance Grant 2024-31Hunan Provincial Department of Finance Grant 2024- 45Hunan Provincial Development and Reform Commission of Innovative Research Program 2021-212-23Natural Science Foundation of Changsha City Kq2403094Natural Science Foundation of Hunan Province 2024JJ5494
6 · The paper itself

Abstract

With the continuous increase of the elderly population and the deepening of population ageing in China, osteoporosis has gradually become one of the significant public health problems. Elucidating the pathophysiological mechanisms that induce osteoporosis and identifying more effective therapeutic targets is of great clinical significance. In this study, in vitro experiments demonstrated that endothelial cell exosomes (EC-EXOs) promoted osteogenic and inhibited adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Aged and ovariectomy (OVX)-induced osteoporosis mice models injected with EC-EXOs confirmed that EC-EXOs delayed bone loss. Proteomic analysis revealed a key protein regulating the differentiation of BMSCs. Expression of THBS3 was significantly higher in EC-EXOs than in Human microvascular endothelial cells (HMEC-1). In vitro and in vivo experiments further validated that THBS3 promoted BMSCs' osteogenic differentiation, inhibited their adipogenic differentiation, and retarded bone loss. Computational biology analysis found that CD47 is a downstream target and potentially functional receptor in BMSCs that bind to THBS3. THBS3 treatment of BMSCs down-regulated the expression of CD47 in in vitro experiments. The aged/OVX models further confirmed that EC-EXOs can regulate the differentiation of BMSCs and delay the process of bone loss via the THBS3-CD47 axis. CD47 antibody may be a potential therapeutic agent for treating ageing-associated bone loss.

Indexed as

CD47 AntigenCell DifferentiationEndothelial CellsMesenchymal Stem CellsOsteoporosisThrombospondinsAdipogenesisAnimalsDisease Models, AnimalFemaleHumansMiceMice, Inbred C57BLOsteogenesisOvariectomyCD47 AntigenThrombospondinsageingbone marrow mesenchymal stem cellsCD47 antibodyosteogenic differentiationosteoporosisthrombospondin‐3vascular endothelial cell exosomes

Identifiers

PMID40514348
PMCPMC12686127

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.