Evidence map›Paper›PMID 40514732›Full record

ArticleChinese medicine2025

Ginsenoside Rg5 alleviates hypoxia-induced myocardial apoptosis by targeting STAT3 to promote Tyr705 phosphorylation.

Fang-Yang Li, Yi-Hao Wang, Cheng Zhang, Wan-Yun Dang, Ze-Kun Wu, Zhen-Hui Wu, Jia-Lu Cui, Xiang-Jun Wu, Chun-Qi Yang, Xue-Cong Tian and 3 more

Abstract read
In one paragraph

Article in Chinese medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Journal of ginseng research · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Fang-Yang Li *School of Pharmacy, Guangdong Pharmaceutical University, Guang Zhou, 510006, China.
Yi-Hao Wang *Beijing Institute of Radiation Medicine, Beijing, 100850, China.
Cheng ZhangSchool of Pharmacy, Guangdong Pharmaceutical University, Guang Zhou, 510006, China.
Wan-Yun DangSchool of Pharmacy, Guangdong Pharmaceutical University, Guang Zhou, 510006, China.
Ze-Kun WuSchool of Pharmacy, Guangdong Pharmaceutical University, Guang Zhou, 510006, China.
Zhen-Hui WuBeijing Institute of Radiation Medicine, Beijing, 100850, China.
Jia-Lu CuiBeijing Institute of Radiation Medicine, Beijing, 100850, China.
Xiang-Jun WuBeijing Institute of Radiation Medicine, Beijing, 100850, China.
Chun-Qi YangBeijing Institute of Radiation Medicine, Beijing, 100850, China.
Xue-Cong TianBeijing Institute of Radiation Medicine, Beijing, 100850, China.
Cheng-Rong XiaoBeijing Institute of Radiation Medicine, Beijing, 100850, China.
Yu-Guang WangBeijing Institute of Radiation Medicine, Beijing, 100850, China. wangyg@bmi.ac.cn.
Yue GaoSchool of Pharmacy, Guangdong Pharmaceutical University, Guang Zhou, 510006, China. gaoyue@bmi.ac.cn.

Funding

he Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (ZYYCXTD-D-202207)the National Natural Science Foundation of China (82204774)
6 · The paper itself

Abstract

backgroundThe heart, as the body's blood-pumping organ, is extremely sensitive to changes in oxygen levels. Myocardial injury caused by hypoxia is a challenging issue, and there are currently no definitive specific drugs available for its treatment. Ginsenoside Rg5, one of the main rare saponins in ginseng, has shown significant efficacy in treating myocardial injury. This study aims to investigate the role and mechanisms of Rg5 in the treatment of hypoxic myocardial injury.

methodsThe cardioprotective effect against acute hypoxia of Rg5 was studied by assessing heart function, myocardial injury markers, inflammation, and oxidative stress in C57 mice, as well as apoptosis and reactive oxygen species (ROS) levels in H9c2 cardiomyocytes. Thermal proteome and target validation techniques were used to confirm the target protein of Rg5. The further protective mechanisms against hypoxia-induced damage were explored using immunocoprecipitation, immunofluorescence and rescue experiments in vivo and in vitro.

resultsThe experimental results demonstrated that Rg5 effectively improved cardiac function in mice, reduced inflammation, oxidative stress, and the release of myocardial injury markers, decreased cardiomyocyte apoptosis, and lowered ROS levels. Further, using target protein screening and validation techniques, Signal transducer and activator of transcription 3 (STAT3) was verified as a direct target for Rg5's cardioprotective effect. It was observed that Rg5 specifically promoted the phosphorylation of Tyr705 in STAT3 via the JAK2/STAT3 pathway, leading to the translocation of phosphorylated STAT3 into the nucleus where they induce the expression of anti-apoptotic protein and protect cells from hypoxic damage.

conclusionRg5 could be a potential therapeutic agent for preventing and treating myocardial hypoxic injury, providing scientific evidence for its application in anti-hypoxic therapy.

Indexed as

Ginsenoside Rg5HypoxiaMyocardial apoptosisStat3Tyr705 phosphorylation

Identifiers

PMID40514732
PMCPMC12166643

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.