ArticlePlanta2025
Integrated transcriptomics and lipidomics reveal mechanisms regulating lipids formation and accumulation in oil body during walnut seed development.
Article in Planta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundThrough combined analysis of the transcriptomics and lipidomics of walnut, the possible molecular mechanism of lipid formation and accumulation in oil bodies was revealed.
conclusionThe formation and accumulation of lipids are critical determinants of nut quality, with walnut storing lipids primarily in oil bodies (OBs). Currently, there is still a lack of systematic research on the formation and accumulation of lipids in walnut OBs (WOBs). Therefore, this study integrated lipidomics and transcriptomics to comprehensively identify the changes in WOBs and walnut kernels at 60, 74, 88, 102, 116, and 130 days after pollination (DAP). The results showed that fatty acid content in walnut kernels and WOBs had opposite trends, especially oleic, linoleic, and linolenic. Principal component analysis of the samples and cluster analysis of differentially expressed genes (DEGs) showed that the total samples were divided into three main groups: 60-74, 88-102, and 116-130 DAP. RNA sequencing generated 33,918 unigenes (14,995 DEGs), including 228 DEGs highly related to lipid metabolism, in 18 cDNA libraries prepared from walnut kernel. These genes were mainly involved in metabolic pathways such as pyruvate metabolism, glycerophospholipid metabolism, glycerolipid metabolism, and fatty acid biosynthesis during lipid synthesis. On the other hand, the expression levels of ACC, KASII, SAD, FAD2, FAD3, and PDAT genes were downregulated at 88-130 DAP compared with 60-74 DAP, which might be the key genes regulating the reduction of free fatty acid content in WOBs. In addition, 21 FAD genes were identified, including seven SAD genes, three FAD2 genes, five FAD3 genes, one FAD5 gene, one FAD6 gene, and four FAD7/8 genes. These genes were closely related to the synthesis of unsaturated fatty acids in WOBs, especially FAD2 and FAD3. The findings offered valuable insights into the dynamic changes in lipids and genetic resources and provided a foundation for walnut quality improvement.
Indexed as
Identifiers
40515948What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.