ArticleMethods in molecular biology (Clifton, N.J.)2025
Activity-Based Protein Profiling of Serine Hydrolases in Bacteria: Methods and Protocols.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Chemoproteomic profiling of adaptive ATP-binding protein activity inFEMS microbes · 2026Article
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Authors and funding
3 authors.
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Abstract
Activity-based protein profiling (ABPP) is a chemoproteomic technique that uses functionalized covalent enzyme inhibitors known as activity-based probes (ABPs) to detect, quantify, and identify active enzymes in a biological sample. Here, we describe experimental protocols for profiling of enzyme activities in bacterial cultures using both fluorescent and biotinylated ABPs that allow for the gel-based detection and resolution of target enzymes, or their enrichment and mass spectrometry-based identification, respectively. These protocols have been optimized for profiling of serine hydrolases using fluorophosphonate probes in both Gram-positive and Gram-negative bacteria, and they can easily be adapted for the application of probes targeting other enzyme families of interest in bacteria under diverse growth conditions.
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Registered trials
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