ArticleResearch and practice in thrombosis and haemostasis2025
Impact of sampling technique, anticoagulant, processing delay, and temperature on murine platelet function in whole blood.
Article in Research and practice in thrombosis and haemostasis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Hemocompatibility of Mechanical Circulatory Support Devices: An Integrated Review of Platelet Function Assessment in In-Vitro Blood Experiments.Annals of biomedical engineering · 2026Review
- Comparative phenotyping of surface markers and glycans in murine and human platelet-derived extracellular vesicles.Research and practice in thrombosis and haemostasis · 2026Article
- Experimental conditions shapeFrontiers in immunology · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Platelets are highly sensitive to subtle changes in their microenvironment, making functional analyses challenging and prone to variation. Advances in understanding how experimental procedures influence human platelet activation have improved the accuracy and comparability of diagnostic and research data. However, despite the pivotal role of murine models, the effects of methodological variations on murine platelets remain incompletely understood. Objectives: To elucidate how blood draw techniques, anticoagulation, processing delay, and assay temperature affect murine platelets. Methods: Blood was obtained by retro-orbital, vena cava, or cardiac puncture and anticoagulated with heparin, citrate, or acid-citrate-dextrose ± recalcification. After 30 to 120 minutes, blood was stimulated at room temperature or 37 °C with adenosine diphosphate (ADP), protease-activated receptor 4-activating peptide (PAR4-AP), or cross-linked collagen-related peptide (CRP-XL), and platelets were analyzed by flow cytometry for CD62P, CD63, CD40L, and activated glycoprotein IIb/IIIa. Results: Blood sampling had minimal impact on ADP-induced platelet activation. However, platelets isolated via vena cava or cardiac puncture exhibited heightened responsiveness to PAR4-AP and CRP-XL, respectively, compared with retro-orbital sampling. Citrate and acid-citrate-dextrose significantly impaired PAR4-AP responses compared with heparin, whereas CRP-XL sensitivity was anticoagulant-independent. Processing delays as brief as 60 minutes significantly altered platelet reactivity to CRP-XL and PAR4-AP, with further delays producing minimal additional impact. Finally, ADP- and CRP-XL-induced platelet activation was significantly reduced at 37 °C compared with room temperature. Conclusion: Common variations in murine platelet handling influence
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