Evidence map›Paper›PMID 40524125›Full record

ArticleJournal of assisted reproduction and genetics2025

Anti-Müllerian hormone binds heat shock protein glucose-regulated protein 78 in human follicular fluid.

Caitlin R Sacha, Nicholas Nagykery, Marie-Charlotte Meinsohn, Karine de Mattos, James A Howard, Hadi Ramadan, Evelyn Minis, LiHua Zhang, Victoria Fitz, Karissa Hammer and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Caitlin R SachaPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA. caitlinsacha@gmail.com.ORCID http://orcid.org/0000-0003-2170-1976
Nicholas NagykeryPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
Marie-Charlotte MeinsohnPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
Karine de MattosPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
James A HowardDepartment of Pharmacology & Systems Physiology, University of Cincinnati, Cincinnati, OH, USA.
Hadi RamadanPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
Evelyn MinisPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
LiHua ZhangPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
Victoria FitzPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
Karissa HammerPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
Irene C SouterMassachusetts General Hospital Fertility Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology, and Reproductive Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Thomas B ThompsonDepartment of Molecular & Cellular Biosciences, University of Cincinnati, Cincinnati, OH, USA.
Patricia K DonahoePediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA.
David PépinPediatric Surgical Research Laboratories and Department of Surgery, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Simches 6, Boston, MA, USA. dpepin@mgh.harvard.edu.ORCID http://orcid.org/0000-0003-2046-6708

Funding

ENVIRONMETAL CARCINOGENESIS AND MUTAGENESIST32ES007250 · NIEHS · UNIVERSITY OF CINCINNATI · PI MILLER, WILLIAM E · 1988 to 2024
$11.9M
Structure-function analysis of Mullerian Inhibiting Substance (MIS)R01HD105818 · NICHD · UNIVERSITY OF CINCINNATI · PI THOMAS B THOMPSON · 2021 to 2026
$2.6M
Methods of fertility protection by Mullerian inhibiting substance in the female reproductive tract during chemotherapyR01HD102014 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI PEPIN, DAVID · 2020 to 2024
$1.8M
Eunice Kennedy Shriver National Institute of Child Health and Human Development 1R01HD102014Eunice Kennedy Shriver National Institute of Child Health and Human Development R01HD105818New England Fertility Society Ferring Grant ME-101649NICHD NIH HHS R01 HD102014NICHD NIH HHS R01 HD105818NIEHS NIH HHS T32 ES007250
6 · The paper itself

Abstract

purposeAnti-Müllerian hormone (AMH) regulates key steps in folliculogenesis, yet unlike other TGF-β family members, no soluble AMH-binding proteins have been identified. We hypothesized that AMH may bind shuttling proteins in human follicular fluid (FF).

methodsDiscarded pooled FF was collected from women undergoing oocyte retrieval at an academic fertility center. AMH-binding soluble proteins in FF were co-immunoprecipitated with AMH, identified by mass spectrometry, and their association was validated by western blot. We characterized the spatiotemporal expression of candidate binding protein glucose-regulated protein 78 (GRP78) in the mouse ovary across the cycle by immunohistochemistry. We used enzyme-linked immunoassay (ELISA) to measure AMH and GRP78 concentrations in patient FF. We used transient transfections in Chinese hamster ovary (CHO) cells to understand the interaction between AMH and GRP78.

resultsCo-immunoprecipitation of AMH in FF identified multiple soluble AMH-binding candidates in the heat-shock protein family, which was confirmed by reciprocal co-immunoprecipitation. We found that GRP78, the most abundant candidate, was enriched in antral follicles of mice at estrus and was present in higher concentrations in human FF than AMH. Finally, we found that AMH overexpression increased endoplasmic reticulum stress and induced GRP78 expression in CHO cells; further ectopic overexpression of GRP78 facilitated the secretion of AMH.

conclusionThis work describes AMH-binding protein candidates identified in human FF and suggests that GRP78 may chaperone AMH during secretion and remain bound in FF. Further study is warranted to understand how heat shock proteins may modulate other biological aspects of AMH and their effects on fertility.

Indexed as

Anti-Mullerian HormoneFollicular FluidHeat-Shock ProteinsAdultAnimalsCHO CellsCricetinaeCricetulusEndoplasmic Reticulum Chaperone BiPEndoplasmic Reticulum StressFemaleHumansMiceOocyte RetrievalOvarian FollicleAnti-Mullerian HormoneEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsHSPA5 protein, humanHspa5 protein, mouseAMHFollicular fluidGRP78/HSPA5HSP90HYOU1IVF

Identifiers

PMID40524125
PMCPMC12289096

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.