Evidence map›Paper›PMID 40524296›Full record

ArticleJournal of traditional Chinese medicine = Chung i tsa chih ying wen pan2025

Identifying potential biomarkers in the hippocampus of chronic fatigue syndrome rats treated with moxibustion at Zusanli (ST36): a proteomics study.

Feng Chuwen, L I Chaoran, Yang Yan, Q U Yuanyuan, Sun Zhongren, Sun Weibo, Liu Tingting, L I Shulin, Yang Tiansong

Abstract read
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In one paragraph

Article in Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Feng ChuwenRehabilitation Department Ⅱ, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine.
L I ChaoranDepartment of Acupuncture, Zhejiang Chinese Medical University, Hangzhou 310000, China.
Yang YanCollege of Basic Medicine of Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Q U YuanyuanRehabilitation Department Ⅱ, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine.
Sun ZhongrenCollege of Basic Medicine of Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Sun WeiboGraduate School of Harbin Medical University, Harbin 150040, China.
Liu TingtingGraduate School of Heilongjiang University of Chinese Medicine, Harbin 150040, China.
L I ShulinRehabilitation Department Ⅱ, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine.
Yang TiansongRehabilitation Department Ⅱ, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine.

Funding

China Postdoctoral Science Foundation: Research on the Mechanism of Moxibustion in Regulating Mitochondrial Autophagy Mediating Microglial Polarization to Promote Synaptic Remodeling in Chronic Fatigue Syndrome Cognitive Impairment 2024MD763980Chunhui Plan of the Ministry of Education: Study on the Mechanism of the Tongdu YuPi Tiaoshen Acupuncture in Improving Hippocampal Synaptic Plasticity of Chronic Fatigue Syndrome Cognitive Impairment HZKY20220308-202201357National Natural Science Foundation of China projects: Research on the Mechanism of Moxibustion Activating Calcium/ Calmodulin-dependent Protein Kinase ⅡPhosphorylation Mediating Long-term Potentiation to Regulate Synaptic Plasticity in the Treatment of Chronic Fatigue Syndrome Cognitive Impairment 82305394Outstanding Youth Project of Heilongjiang Provincial Natural Science Foundation: the Mechanism of Moxibustion in Improving Chronic Fatigue Syndrome Cognitive Impairment by Regulating Calcium/Calmodulin-dependent Protein Kinase II-mediated Long-Term Potentiation YQ2023H019Post-doctoral Program of Heilongjiang Province: based on the Calcium/Calmodulin-dependent Protein Kinase II/Parkin/NLR Family Pyrin Domain Containing 3 Signaling Axis to Explore the Mechanism of Moxibustion in Mitochondrial Autophagy Mediating Microglial Polarization to Promote Synaptic Remodeling in Chronic Fatigue Syndrome Cognitive Impairment LBH-TZ2420Research on the Mechanism of Electroacupuncture Regulating Murine Double Minute 2 Ubiquitination Postsynaptic Density Protein 95 Levels to Reshape the Synaptic Structure of Hippocampal Neurons and Improve Chronic Fatigue Syndrome Cognitive Impairment 82074539Research Project of Traditional Chinese Medicine in Heilongjiang Province: Study on the Mechanism of Acupuncture in Improving Hippocampal Synaptic Plasticity of Chronic Fatigue Syndrome Cognitive Impairment ZHY2022-136Study on the Mechanism of Moxibustion at the Zusanli (ST36) Regulating Long-Term Potentiation to Promote Synaptic Remodeling and Improve Cognitive Impairment in Chronic Fatigue Syndrome LBH-Z23281Youth Talent Support Project of the Chinese Association of Traditional Chinese Medicine: Research on the Molecular Pathways of Moxibustion at the Zusanli (ST36) Point in Regulating Synaptic Plasticity to Improve Chronic Fatigue Syndrome Cognitive Impairment 2023-QNRC2-A04Youth Talent Support Project of the Heilongjiang Provincial Association of Traditional Chinese Medicine: Study on the Effect and Mechanism of Tongdu Yupi Tiaoshen Acupuncture on Improving Hippocampal Synaptic Plasticity in Chronic Fatigue Syndrome Cognitive Impairment 2022-QNRC1-05
6 · The paper itself

Abstract

objectiveTo observe the effects of moxibustion at Zusanli (ST36) on rats with chronic fatigue syndrome (CFS) and to analyze the mechanisms of moxibustion through hippocampal Proteomics.

methodsMale Sprague-Dawley (SD) rats were randomly divided into three groups: control group (CON), model group (MOD), and moxibustion group (MOX), with 12 rats in each group. The MOD and MOX groups underwent chronic multi-factor stress stimulation for 35 d to establish the CFS model. After modeling, the rats in the MOX group received mild moxibustion at Zusanli (ST36) (bilateral) for 10 minutes daily for 28 d. During the treatment period, rats in both the MOD and MOX groups continued modeling, while the CON group was kept under normal breeding conditions. The general condition of the rats was monitored, and behaviors were assessed using the Open Field Test (OFT), Exhaustion Treadmill Test, and Morris Water Maze (MWM). Hematoxylin and eosin (HE) staining and transmission electron microscopy (TEM) were employed to observe morphological changes in the hippocampus. Label-free Proteomics were utilized to identify differentially expressed proteins (DEPs) in the hippocampus, followed by bioinformatics analysis. The reliability of the Proteomics results was verified using Parallel Reaction Monitoring.

resultsA: Moxibustion at Zusanli (ST36) significantly reduced the general condition score of CFS rats, improved their behavioral performance in OFT, treadmill and MWM, and repaired the pathological and synaptic structural damage in the hippocampus.B: We identified DEPs by applying a fold change threshold of 1.2 and a significance level of

conclusionMoxibustion at Zusanli (ST36) is effective in treating CFS, the potential biomarkers identified by Proteomics confirm that the mechanisms of moxibustion involve multiple targets and pathways, which may be key to regulating the structural and functional damage in the hippocampus associated with CFS, highlighting their significant value for future research.

Indexed as

Acupuncture PointsFatigue Syndrome, ChronicHippocampusMoxibustionAnimalsBiomarkersHumansMaleProteomicsRatsRats, Sprague-DawleyBiomarkerscomputational biologyfatigue syndrome, chronicmoxibustionpoint ST36 (Zusanli)proteomics

Identifiers

PMID40524296
PMCPMC12138249

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.