Evidence mapPaperPMID 40526038Full record

ArticlePhysiological reports2025

Short-term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice.

Marissa I McGilvrey, Bethany Fortier, Diana Cooke, Maryam A Mahdi, Benjamin Tero, Christian M Potts, Abigail Kaija, Larisa Ryzhova, Carolina Cora, Adam Richardson and 6 more

Abstract read
In one paragraph

Article in Physiological reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Marissa I McGilvreyCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Bethany FortierCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Diana CookeOrentreich Foundation for the Advancement of Science, Inc., Cold Spring, New York, USA.
Maryam A MahdiCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Benjamin TeroCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Christian M PottsCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Abigail KaijaCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Larisa RyzhovaCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Carolina CoraCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.ORCID https://orcid.org/0000-0002-7877-0297
Adam RichardsonPanome Bio, St. Louis, Missouri, USA.
Douglas GuziorPanome Bio, St. Louis, Missouri, USA.
Ilka PinzCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Calvin VaryCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Robert A KozaCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.
Gene AblesOrentreich Foundation for the Advancement of Science, Inc., Cold Spring, New York, USA.
Lucy LiawCenter for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, USA.ORCID https://orcid.org/0000-0003-3341-0156

Funding

Translational Research Technologies CoreU54GM115516 · MAINEHEALTH · 2025 to 2025
$4.0M
The role of night shift work in metabolic disorders during and after pregnancyP20GM121301 · MAINEHEALTH · 2025 to 2025
$2.6M
Regulation of arterial phenotype by perivascular adipose tissue in cardiometabolic diseaseR01HL141149 · MAINEHEALTH · 2025 to 2025
$574k
American Heart Association (AHA) 19TPA34850041American Heart Association (AHA) 23PRE1022890National Heart Lung and Blood Institute R01HL141149NHLBI NIH HHS R01 HL141149NIGMS NIH HHS 5P20GM12130NIGMS NIH HHS P20 GM121301NIGMS NIH HHS U54 GM115516NIGMS NIH HHS U54GM115516
6 · The paper itself

Abstract

Dietary methionine restriction (MetR) promotes metabolic health, and we tested the impact of short durations of MetR on high fat diet (HFD)-induced metabolic dysfunction with the maintenance of HFD. Male C57BL/6J mice were fed HFD from 10 to 25 weeks of age, then maintained on HFD or fed HFD with 80% reduced methionine (HFD-MetR) for 3, 5, or 10 days. Blood, liver, adipose tissue, and aortae underwent phenotypic assessment, proteomics, and metabolomics. HFD-MetR induced rapid weight loss and robust metabolic improvement within 10 days. Significant reductions in body weight, circulating triglycerides, glucose, insulin, adipokines and hepatokines reflected metabolic health. Proteomics revealed enriched metabolic signatures in perivascular adipose tissue (PVAT) and structural remodeling signatures in aorta. Metabolomics identified a cardioprotective signature in blood plasma, and activated mitochondrial activity and energy production in liver and brown adipose tissue. HFD-MetR reversed metabolic dysfunction, and novel proteomic and metabolomic signatures were identified. Multi-organ molecular changes in lipid metabolism, mitochondrial function, and bioenergetics are predicted to impact adipose tissue and liver function and cardiovascular health. Our identification of rapid changes in protein and metabolite signatures with accelerated restoration of metabolic health can be leveraged to evaluate biomarkers of metabolic health and disease in a translational context.

Indexed as

Diet, High-FatMetabolic DiseasesMethionineAdipose TissueAnimalsEnergy MetabolismLipid MetabolismLiverMaleMiceMice, Inbred C57BLMethionineadipose tissuehigh fat dietmetabolomicsmethionine restrictionproteomics

Identifiers

PMID40526038
PMCPMC12172570

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.