ArticleAnalytical chemistry2025
Single-Cell Lipidomics by LC-MS Interlaboratory Study Reveals the Impact of X-ray Irradiation on a Pancreatic Cancer Cell Line and Its Bystanders.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Imaging-Guided Live Single-Cell Lipid Profiling of Leader and Follower Cells During Collective Migration of Triple-Negative Breast Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cellular level lipidomics of two-dimensional cultures of adherent gut epithelial cell lines confirms a metabolic switch.The Analyst · 2026Article
- Single-cell metabolome and RNA-seq multiplexing on single plant cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live single-cell lipidomics by liquid chromatography mass spectrometry (LC-MS) is a nascent and rapidly growing field which can shed new light on infectious diseases, cancer, immunology, and drug delivery. There are now a growing number of laboratories that can isolate single cells and laboratories that can perform lipidomics analysis at correspondingly low sample volumes, but there is a lack of validation data. We have carried out the first interlaboratory LC-MS lipidomics experiment for single cells, aimed at filling this gap. We present a novel workflow to enable interlaboratory studies, comprising live-cell imaging and single-cell isolation, followed by freeze-drying, international shipping, reconstitution, and untargeted lipidomics analysis. We applied this methodology to reveal radiation-induced bystander effects in pancreatic cancer cells. X-ray irradiated cells and their bystanders sampled live 48 h postirradiation demonstrated reduced lipid abundance compared to controls, with distinct changes in molar ratios of several polyunsaturated lipids. This demonstrates for the first time that radiation can cause considerable cellular lipid remodelling, not only at the site of delivery. A striking similarity in lipid changes was observed between the two participating laboratories despite differences in sample preparation and analysis methods. Our results are further corroborated by live-cell imaging analysis of lipid droplets. This work serves as an important validation and demonstration of the nascent and rapidly growing field of live single-cell lipidomics.
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Registered trials
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