Evidence mapPaperPMID 40530696Full record

ArticleNucleic acids research2025

Replication stress responses in human lymphocytes change sex-specifically during aging.

Melanie Rall-Scharpf, Dominik Schlotter, Philipp Koch, Karol Szafranski, Marco Groth, Arne Sahm, Stephanie Biber, Bryan A Castaño, Benedikt Heitmeir, Friedemann Zengerling and 7 more

Abstract read
In one paragraph

Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Melanie Rall-ScharpfDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.ORCID 0000-0003-2991-5223
Dominik SchlotterDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.ORCID 0009-0008-2814-9444
Philipp KochLeibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany.ORCID 0000-0003-2825-7943
Karol SzafranskiLeibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany.
Marco GrothLeibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany.ORCID 0000-0002-9199-8990
Arne SahmLeibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany.ORCID 0000-0002-7330-1790
Stephanie BiberDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.
Bryan A CastañoDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.
Benedikt HeitmeirDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.
Friedemann ZengerlingDepartment of Urology, Ulm University, 89081 Ulm, Germany.
Miriam DenizDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.
Dhayana DallmeierInstitute for Geriatric Research, Ulm University Medical Centre at Agaplesion Bethesda Clinic, 89073 Ulm, Germany.
Stefanie BraigInstitute of Epidemiology and Medical Biometry, Ulm University, 89081 Ulm, Germany.
Halvard BonigInstitute for Transfusion Medicine and Immunohematology, Faculty of Medicine, Goethe University, and German Red Cross Blood Service, 60528 Frankfurt, Germany.
Michael MilyavskyDepartment of Pathology, Faculty of Medical and Health Sciences, Tel-Aviv University, 69978 Tel-Aviv, Israel.ORCID 0000-0002-5083-0809
Helmut PospiechLeibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany.
Lisa WiesmüllerDepartment of Obstetrics and Gynecology, Ulm University, 89075 Ulm, Germany.ORCID 0000-0002-2397-5041

Funding

ActiFE study DE2674/1-1ActiFE study RO2606/14-1B3 in Collaborative Research Center 1506 450627322Cellular and Molecular Mechanisms in Aging 194266605Else Kröner Fresenius FoundationFederal Government of GermanyGerman Israeli Foundation I.-1304-412.13/2015German Research FoundationStart-up 194266605State of Thuringia
6 · The paper itself

Abstract

The varying incidence of aging-related diseases and the gender gap in life expectancy suggest differences in the aging process between the sexes. Yet, little is known about sex-specific differences in genomic instability, a key factor in aging. Here, we analyzed DNA damage responses (DDRs) in cycling peripheral blood lymphocytes (PBL) and hematopoietic stem and progenitor cells (HSPC) from female and male donors of different age, focusing on replication stress. Transcriptomics revealed striking sex-dependent expression changes in DDR pathways during aging. Particularly, various DDR components, involved in DNA repair and replication fork remodeling, were upregulated with age in men. In older women, functional analysis indicated reduced activity of the Fanconi anemia pathway. Analyses of replication dynamics, PCNA ubiquitination, translesion synthesis (TLS)-polymerase signals, and sensitivities to TLS-polymerase inhibitors indicate a shift from fork remodeling to fast TLS inducing nonclassical replication stress. While replication dynamics were unaltered and replication stress rather reduced, PBL from older men were highly dependent on PARP activity. In conclusion, our findings revealed sex-specific strategies to cope with replication stress in PBL from older individuals, namely through DNA damage tolerance pathway switching in women and PARP activation in men, differentially contributing to the decline of genomic stability with age.

Indexed as

AgingDNA ReplicationLymphocytesAdultAgedAged, 80 and overDNA DamageDNA RepairFemaleGenomic InstabilityHematopoietic Stem CellsHumansMaleMiddle AgedProliferating Cell Nuclear AntigenSex CharacteristicsProliferating Cell Nuclear Antigen

Identifiers

PMID40530696
PMCPMC12203915

What Socratic holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.