Evidence map›Paper›PMID 40530826›Full record

ReviewGut microbes2025

Being a better version of yourself: genetically engineered probiotic bacteria as host defense enhancers in the control of intestinal pathogens.

Ewa Carolak, Joanna Czajkowska, Adrianna Stypułkowska, Wiktoria Waszczuk, Agata Dutkiewicz, Krzysztof Grzymajlo

Abstract readReview
In one paragraph

Review in Gut microbes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Review
  2. Review
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  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Bacteriocins fromFrontiers in microbiology · 2026
    Review
  14. Vitamin D3 contribution to systemic and local control ofFrontiers in molecular biosciences · 2026
    Article
  15. Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ewa CarolakFaculty of Veterinary Medicine, Department of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.ORCID 0000-0002-1762-7796
Joanna CzajkowskaFaculty of Veterinary Medicine, Department of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.ORCID 0000-0002-6750-409X
Adrianna StypułkowskaFaculty of Veterinary Medicine, Department of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.ORCID 0000-0002-5245-9206
Wiktoria WaszczukFaculty of Veterinary Medicine, Department of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.ORCID 0000-0002-5318-6107
Agata DutkiewiczFaculty of Veterinary Medicine, Department of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.ORCID 0000-0001-5506-4114
Krzysztof GrzymajloFaculty of Veterinary Medicine, Department of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.ORCID 0000-0002-1163-0679

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intestinal pathogens pose a significant global health burden, and traditional antibiotic treatments often disrupt the beneficial gut microbiota that plays a crucial role in maintaining host health through pathogen prevention and immune regulation. Although probiotics have emerged as promising therapeutic agents, their efficacy is limited by strain-dependent variations, survival challenges in the gastrointestinal tract, and inconsistent immune responses. Recent advances in genetic engineering, particularly CRISPR-Cas systems and their combinations with complementary technologies, such as Cre-lox and RecE/T, have enabled the precise modification of probiotic strains to enhance their therapeutic potential. These enhanced probiotics demonstrate improved functionality through multiple mechanisms, including increased adhesion via the expression of specific proteins (InlA, FnBPA, and LAP), targeted antimicrobial activity through engineered sensing systems (

Indexed as

BacteriaGastrointestinal MicrobiomeMicroorganisms, Genetically-ModifiedProbioticsAnimalsGenetic EngineeringHumansbiosafetyCRISPR-Casgastrointestinal pathogensgenetic modificationgut healthmicrobiomeProbiotics

Identifiers

PMID40530826
PMCPMC12184120

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.