Evidence map›Paper›PMID 40531456›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Step-by-Step Guide for Biosynthesis of Recombinant Fusion Antimicrobial Peptide and Release of the Active Peptide from Its Fusion Partner by Formic Acid Cleavage.

Sivakumar Jeyarajan, Sukumar Ranjith, Bency Thankappan, Anbarasu Kumarasamy

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sivakumar JeyarajanMicrobial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Sukumar RanjithMicrobial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Bency ThankappanMicrobial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Anbarasu KumarasamyMicrobial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India. anbumbt@bdu.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter explains the step-by-step protocol for cloning the gene for short peptides with a fusion partner and the biosynthesis of recombinant fusion peptide in Escherichia coli followed by the separation of the active peptide by formic acid cleavage and its purification and characterization. The advantage of formic acid cleavage is its cost-effectiveness in releasing the active peptides from the fusion partner compared to enzymatic cleavage and the nonrequirement of folded conformation for acid cleavage. This reduces the time, labor, and steps required for removing the denaturant, thus simplifying the procedure. With this procedure, 6.5 mg/L of recombinant fusion peptide was obtained. The challenges associated with acid digestion are also mentioned in this protocol.

Indexed as

Antimicrobial PeptidesFormatesRecombinant Fusion ProteinsCloning, MolecularEscherichia coliAntimicrobial PeptidesFormatesformic acidRecombinant Fusion ProteinsCloningFormic acid cleavageGenetic engineeringProtein expression optimizationSDS-PAGETherapeutic antimicrobial peptidesThioredoxin

Identifiers

PMID40531456

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.