Evidence map›Paper›PMID 40532039›Full record

ArticlePloS one2025

Protection of LPS-induced intestinal injury in goslings by polysaccharide of Atractylodes macrocephala Koidz based on 16S rRNA and metabolomics analysis.

Wanyan Li, Baili Lu, Shirou Pan, Shuaifei Bai, Jiayu He, Bingxin Li, Nan Cao, Xinliang Fu, Junhao Wei, Ying Chen and 3 more

Expression of concernAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Modulation of the gut microbiota by traditional Chinese medicine to attenuate chemotherapy-induced toxicity in gastric cancer: a review.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2026
    Review
  3. Article
  4. Article
  5. Polysaccharides fromNutrients · 2025
    Review
  6. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Wanyan LiCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Baili LuCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Shirou PanCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Shuaifei BaiCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Jiayu HeCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Bingxin LiCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Nan CaoCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Xinliang FuCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Junhao WeiFaculty of Applied Sciences, Macao Polytechnic University, Macao, China.ORCID 0009-0006-0553-2032
Ying ChenDepartment of Health Management, School of Health Economics and Management, Nanjing University of Chinese Medicine, Nanjing, China.
Yunmao HuangCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Yunbo TianCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Danning XuCollege of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.ORCID 0000-0003-2958-1825

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The protective effects of the polysaccharide of Atractylodes macrocephala Koidz (PAMK) against lipopolysaccharide (LPS)-induced intestinal injury in goslings was determined using 16S rRNA analysis of cecal contents and serum metabolomics analysis. PAMK was administered to goslings following LPS-induced intestinal injury, and its effects were assessed. PAMK significantly reduced the serum levels of inflammatory factors including interleukin (IL)-6 and C-reactive protein and decreased the expression of pro-inflammatory cytokines including interleukin(IL)-1[Formula: see text], IL-6, and toll-like receptor 2 in jejunal tissues. Moreover, PAMK significantly upregulated the relative mRNA expression levels of the tight junction proteins Zonula occludens-1, Occludin, Claudin, and Mucin-2, enhancing the integrity of the intestinal barrier and alleviating LPS-induced intestinal injury. 16S rRNA sequencing revealed that PAMK could alleviate LPS-induced disruption of the intestinal microbiota structure and improve microbial diversity. Metabolomics analysis revealed that PAMK could influence key metabolic pathways, including the mTOR, PI3K-Akt, and FoxO signaling pathways, and regulate metabolites such as L-aspartic acid and S-adenosylmethionine. Integrated analysis indicated that PAMK could promote the enrichment of beneficial bacteria (e.g., Allobaculum and Peptococcus) while alleviating LPS-induced microbial dysbiosis by modulating the correlation between key metabolites and specific microbial populations. Overall, PAMK could alleviate LPS induced intestinal injury by enhancing intestinal barrier function, optimizing gut microbiota composition, and regulating metabolic signaling pathways. Our findings provide a novel strategy for maintaining the intestinal health of poultry and preventing intestinal diseases.

Indexed as

AtractylodesIntestinesLipopolysaccharidesPolysaccharidesRNA, Ribosomal, 16SAnimalsGastrointestinal MicrobiomeIntestinal MucosaMetabolomicsSignal TransductionLipopolysaccharidesPolysaccharidesRNA, Ribosomal, 16S

Identifiers

PMID40532039
PMCPMC12176298

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.