Evidence mapPaperPMID 40537599Full record

ArticleBiological trace element research2026

Zinc Alleviates Oxidative Stress Injury of the Testis Induced by Zearalenone through Sirt3-Foxo3 Pathway in Mice.

Zhifang Zhao, Jibin Yao, Zhong Guo, Lei Song, Jianxiu Ma, Yihang Song, Yanqing Ma, Jin Zhao

Abstract read
In one paragraph

Article in Biological trace element research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhifang Zhao *College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730030, China.
Jibin Yao *Department of Surgical Oncology, Gansu Province Hospital, Lanzhou, China.
Zhong GuoMedical College, Northwest Minzu University, Lanzhou, China.
Lei SongMedical College, Northwest Minzu University, Lanzhou, China.
Jianxiu MaMedical College, Northwest Minzu University, Lanzhou, China.
Yihang SongCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730030, China.
Yanqing MaMedical College, Northwest Minzu University, Lanzhou, China.
Jin ZhaoCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730030, China. yxzj@xbmu.edu.cn.

Funding

Fundamental Research Funds for the Central Universities Grant No.31920220114Gansu Province Higher Education Institutions Innovation Foundation Project Grant No.2022B-064Natural Science Foundation of Gansu Province Grant No.23JRRA1738Research project of Innovation and Entrepreneurship Education Reform in Northwest Minzu University Grant No. 2022XJCXCYJGXM14Virtual Simulation Experimental Teaching First Class Course Grant No.2021XJYLKC-06
6 · The paper itself

Abstract

Environmental pollutants, such as zearalenone (ZEA), a mycotoxin from Fusarium graminearum that contaminates cereal crops and animal feed, significantly threaten reproductive health. The main toxicity mechanism of ZEA involves triggering oxidative stress and apoptosis. Zinc (Zn), a critical antioxidant for reproductive health, may counteract ZEA toxicity, but its mechanism of action remains unclear. The objective of the study was to investigate the alleviating effects of Zn supplementation against ZEA-induced testicular toxicity. We combined network toxicology and animal experiments to evaluate Zn's efficacy. Male Kunming mice were divided into Control, ZEA (2 mg/kg), Zn (20 mg/kg) and ZEA + Zn (10/20/40 mg/kg) groups and treated for 28 days. The results revealed that ZEA exhibited marked reductions in sperm quality, accompanied by structural testicular injury to the testis. ZEA diminishes the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), while simultaneously increasing the levels of malondialdehyde (MDA), which results in oxidative stress. ZEA down-regulates serum levels of testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and impairs steroidogenesis. Significant elevations in ROS levels and apoptotic protein expression were observed in the testicular tissue of the ZEA group. ZEA significantly decreased both the mRNA and protein expression levels of Sirt3 and Foxo3, as well as their immunofluorescence intensity. Zn significantly increased sperm quality, improved testicular morphology, increased Sirt3 and Foxo3 levels, reduced ROS, normalized antioxidant capacity, improved apoptosis, and restored serum hormone levels. These findings emphasize the reproductive risks associated with ZEA, while also highlighting Zn as a possible candidate for developing alleviating effects against mycotoxin-induced reproductive toxicity.

Indexed as

Forkhead Box Protein O3Oxidative StressSirtuin 3TestisZearalenoneZincAnimalsAnimals, Outbred StrainsMaleMiceForkhead Box Protein O3FoxO3 protein, mouseSirt3 protein, mouseSirtuin 3ZearalenoneZincOxidative stressSirt3-Foxo3Testicular injuryZearalenoneZinc

Identifiers

PMID40537599
PMCPMC12847136

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.