Evidence map›Paper›PMID 40537734›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2025

Mass Spectrometry-Based Untargeted Metabolomics Identifies Distinct Metabolic Signatures in Infertility: A Comparative Analysis of PCOS, POR, and NOR.

Metin Demirel, Mehtap Alim, Fatmanur Koktasoglu, Nil Atakul, Ebru Guner, Ayse Nur Işık Aydın, Sanem Naz Kafali, Yildız Atamer, Sahabettin Selek

Abstract readComparative Study
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Metin DemirelDepartment of Medical Biochemistry, Bezmialem Vakif University Faculty of Medicine, 34093, Istanbul, Turkey. medemirel@bezmialem.edu.tr.ORCID 0000-0003-4810-6771
Mehtap AlimDepartment of Medical Biochemistry, Bezmialem Vakif University Faculty of Medicine, 34093, Istanbul, Turkey.ORCID 0000-0003-0818-7714
Fatmanur KoktasogluDepartment of Medical Biochemistry, Bezmialem Vakif University Faculty of Medicine, 34093, Istanbul, Turkey.ORCID 0000-0002-3201-5923
Nil AtakulDepartment of Gynaecology and Obstetrics, Istanbul Education Research Hospital, 34093, Istanbul, Turkey.ORCID 0000-0003-3874-8797
Ebru GunerDepartment of Gynaecology and Obstetrics, Istanbul Education Research Hospital, 34093, Istanbul, Turkey.
Ayse Nur Işık AydınDepartment of Gynaecology and Obstetrics, Istanbul Education Research Hospital, 34093, Istanbul, Turkey.
Sanem Naz KafaliÜsküdar American Academy, Istanbul, Turkey.
Yildız AtamerDepartment of Medical Biochemistry, Faculty of Medicine, Beykent University, 34093, Istanbul, Turkey.ORCID 0000-0002-0934-4278
Sahabettin SelekDepartment of Medical Biochemistry, Bezmialem Vakif University Faculty of Medicine, 34093, Istanbul, Turkey.ORCID 0000-0003-1235-3957

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundInfertility affects approximately 15% of reproductive-age couples, with polycystic ovary syndrome and poor ovarian reserve being major contributing factors. Metabolomic profiling of follicular fluid offers insights into the underlying metabolic disturbances associated with these infertility phenotypes. This study aims to identify metabolic biomarkers distinguishing PCOS, POR, and male factor infertility, which may facilitate improved diagnostic and therapeutic strategies.

methodsA total of 119 participants were categorized into three groups: PCOS (n = 39), POR (n = 40), and NOR (n = 40). Liquid chromatography-high-resolution mass spectrometry was used for untargeted metabolomic profiling. Metabolites were identified using HMDB, MassBank, and MoNA, while pathway analysis was performed using KEGG. Statistical analyses were conducted using R and Python, including one-way ANOVA, t-tests, and Mann-Whitney U tests, with False Discovery Rate correction applied.

resultsDistinct metabolic alterations were observed among the groups. Trehalose-6-phosphate, taurocholate, and N,N-dimethylglycine emerged as the most significantly altered metabolites, showing strong discriminatory potential between PCOS and POR. PCOS patients exhibited reduced levels of taurocholate, mycalemide, and trehalose-6-phosphate, whereas NOR patients showed elevated levels of N,N-dimethylglycine and argininosuccinate. The POR group demonstrated increased levels of 1-methyl-2-pyrrolidone and haplopine, along with a broader metabolite distribution.

conclusionThis study reveals phenotype-specific metabolic signatures in PCOS and POR, identifying taurocholate, mycalemide, and N,N-dimethylglycine as potential follicular biomarkers. These findings contribute to a deeper understanding of the metabolic basis of infertility and highlight the potential of follicular fluid metabolomics for precision medicine in reproductive health.

Indexed as

Infertility, FemaleInfertility, MaleMetabolomeMetabolomicsPolycystic Ovary SyndromeAdultBiomarkersFemaleFollicular FluidHumansMaleMass SpectrometryYoung AdultBiomarkersFollicular fluidInfertilityMetabolic pathwaysPolycystic ovary syndromePoor ovarian reserveTaurocholateTrehalose-6-phosphateUntargeted metabolomics

Identifiers

PMID40537734
PMCPMC12271243

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.