Evidence mapPaperPMID 40537735Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2025

Role of Defense/Immunity Proteins in Non-Obstructive Azoospermia: Insights from Gene Expression and Single-Cell RNA Sequencing Analyses.

Ali Shakeri Abroudi, Hossein Azizi, Melika Djamali, Hewa Khalid Abdullah, Ali Qorbanee, Thomas Skutella

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Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Ali Shakeri AbroudiDepartment of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Hossein AziziFaculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran. H.azizi@ausmt.ac.ir.ORCID 0000-0001-8246-595X
Melika DjamaliDepartment of Biology, Faculty of Science, Tehran University, Tehran, Iran.
Hewa Khalid AbdullahDepartment of Surgery, Faculty of General of Medicine, Koya University, Kurdistan Region - F.R, Koya, KOY45, Iraq.
Ali QorbaneeDepartment of Surgery, Faculty of General of Medicine, Koya University, Kurdistan Region - F.R, Koya, KOY45, Iraq.
Thomas SkutellaInstitute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120, Heidelberg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-obstructive azoospermia (NOA) is a severe form of male infertility characterized by a complete absence of sperm in the ejaculate due to impaired spermatogenesis. While genetic and hormonal factors are known contributors, recent evidence highlights the role of immune dysregulation in NOA pathophysiology. Defense/immunity proteins play a crucial role in maintaining testicular immune homeostasis, but their aberrant expression may contribute to spermatogenic failure. This study analyzed gene expression data from multiple datasets to identify immune-related genes significantly altered in NOA, including IFITM1, CES1, MR1, LSAMP, PRB2, CARD8, and PECAM1. Differential expression analysis revealed upregulation of IFITM1 and PECAM1 and downregulation of CES1, MR1, LSAMP, PRB2, and CARD8, suggesting immune imbalance and oxidative stress contribute to NOA pathogenesis. Additionally, single-cell RNA sequencing confirmed their differential expression in distinct testicular cell populations. Our findings suggest that immune-related pathways, including cytokine signaling, antigen processing, and oxidative stress response, may contribute to NOA pathogenesis. Targeting these molecular pathways could provide novel therapeutic strategies for improving reproductive outcomes in affected individuals.

Indexed as

AzoospermiaTestisHumansMaleOxidative StressSequence Analysis, RNASingle-Cell AnalysisDefense/immunity proteinsMale infertilityNon-obstructive azoospermiaSpermatogenesisTesticular immune response

Identifiers

PMID40537735

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.