Evidence mapPaperPMID 40539039Full record

ArticleFrontiers in immunology2025

Identification of the microglia-associated signature in experimental autoimmune encephalomyelitis.

Yan Wu, Jianhong Wang, Bo Chen, Yuxue Guo, Ping Gan, Yanbing Han

Abstract read
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yan WuNeurology Department, First Affiliated Hospital of Kunming Medical University, Kunming, China.
Jianhong WangNeurology Department, First Affiliated Hospital of Kunming Medical University, Kunming, China.
Bo ChenDepartment of Neurology, Tongji Hospital of Tongji Medical College, Huazhong University of Science of Technology, Wuhan, China.
Yuxue GuoNeurology Department, First Affiliated Hospital of Kunming Medical University, Kunming, China.
Ping GanBiochemistry and Molecular Department, College of Basic Medicine, Kunming Medical University, Kunming, Yunnan, China.
Yanbing HanNeurology Department, First Affiliated Hospital of Kunming Medical University, Kunming, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Multiple sclerosis (MS) is a chronic neuroinflammatory disorder characterized by demyelination and immune dysregulation, and microglia play a central role in disease progression. Despite this, the specific microglial gene signatures contributing to MS remain inadequately characterized. Methods: We utilized an experimental autoimmune encephalomyelitis (EAE) mouse model and performed RNA sequencing to identify differentially expressed Messenger RNAs (DEmRNAs), Long Non-Coding RNAs (DElncRNAs), Circular RNAs (DEcircRNAs), and microRNAs (DEmiRNAs) in microglia. A machine learning approach incorporating five distinct algorithms was applied to select a robust multigene signature. The biological functions of the included genes were assessed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and validated by quantitative reverse transcription PCR (qRT-PCR). Additionally, molecular docking studies were conducted to explore potential interactions with approved MS therapeutics. Results: Six DEmRNAs were identified as key microglia-associated biomarkers: Neutrophilic Granule Protein ( Conclusions: This study provides a comprehensive microglial gene signature for EAE, highlighting the involvement of TLR pathways and circRNA-mediated regulation in MS pathogenesis. These findings provide a foundation for future research into microglia-targeted therapies and diagnostic tools for MS.

Indexed as

Encephalomyelitis, Autoimmune, ExperimentalMicrogliaTranscriptomeAnimalsBiomarkersDisease Models, AnimalFemaleGene Expression ProfilingMiceMice, Inbred C57BLMicroRNAsMultiple SclerosisBiomarkersMicroRNAsexperimental autoimmune encephalomyelitis (EAE)machine learning (ML)microgliamultiple sclerosis (MS)RNA sequencing

Identifiers

PMID40539039
PMCPMC12176564

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.