Evidence map›Paper›PMID 40542254›Full record

ArticleNpj viruses2025

Longitudinal kinetics of the viral infection biomarker 3'-deoxy-3',4'-didehydro-cytidine in SARS-CoV-2, influenza A virus and RSV human challenge models.

Ravi Mehta, Elena Chekmeneva, Stephanie Ascough, Helen Wagstaffe, Loukas Papargyris, Malick Gibani, Ada H Y Yuen, Maria Valdivia-Garcia, Caroline Sands, María Gómez-Romero and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Npj viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Ravi MehtaDepartment of Infectious Disease, Imperial College London, London, UK.
Elena ChekmenevaNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
Stephanie AscoughDepartment of Infectious Disease, Imperial College London, London, UK.
Helen WagstaffeDepartment of Infectious Disease, Imperial College London, London, UK.
Loukas PapargyrisDepartment of Infectious Disease, Imperial College London, London, UK.
Malick GibaniDepartment of Infectious Disease, Imperial College London, London, UK.
Ada H Y YuenNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
Maria Valdivia-GarciaNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
Caroline SandsNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
María Gómez-RomeroNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
Ewurabena A MillsDepartment of Infectious Disease, Imperial College London, London, UK.
Vincenzo SgroDepartment of Infectious Disease, Imperial College London, London, UK.
Lydia SlaterDepartment of Infectious Disease, Imperial College London, London, UK.
Pete DayanandaDepartment of Infectious Disease, Imperial College London, London, UK.
Claire BroderickDepartment of Infectious Disease, Imperial College London, London, UK.
Jiayun XuDepartment of Infectious Disease, Imperial College London, London, UK.
Myrsini KaforouDepartment of Infectious Disease, Imperial College London, London, UK.
Lynn MaslenNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
Mahdad NoursadeghiDivision of Infection and Immunity, University College London, London, UK.
Andrew J PollardOxford Vaccine Group, Department of Paediatrics, and the NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, UK.
Wendy BarclayDepartment of Infectious Disease, Imperial College London, London, UK.
Zoltan TakatsNational Phenome Centre, Section of Bioanalytical Chemistry, Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Hospital Campus, IRDB Building, London, UK.
Christopher ChiuDepartment of Infectious Disease, Imperial College London, London, UK. c.chiu@imperial.ac.uk.
Shiranee SriskandanDepartment of Infectious Disease, Imperial College London, London, UK. s.sriskandan@imperial.ac.uk.

Funding

Wellcome Trust
6 · The paper itself

Abstract

3'-deoxy-3',4'-didehydro-cytidine (ddhC) is a recently discovered host biomarker for viral infections, though its temporal kinetics remain unclear. This study tests the hypothesis that ddhC is an acute phase reactant, rising shortly after viral infection and subsequently falling to baseline. We leveraged the precise monitoring facilitated by human challenge studies to investigate healthy participants inoculated with SARS-CoV-2, influenza A virus (H3N2), or respiratory syncytial virus (RSV). Using targeted liquid chromatography-tandem mass spectrometry, we quantified ddhC concentrations in serial plasma samples collected pre- and post-inoculation. In SARS-CoV-2 and H3N2 influenza A virus infection, but not RSV, ddhC levels peaked at 3-7 days post inoculation and declined to baseline by days 10-14. This pattern was also observed in asymptomatic or paucisymptomatic participants. A comparison of ddhC concentrations with matched timepoint whole blood gene expression revealed a correlation with interferon-related genes, including viperin and CMPK2-enzymes implicated in its upstream biosynthesis. Our results suggest that ddhC is a biomarker of the acute phase of viral infection, with potential to guide early interventions that reduce antimicrobial resistance and strengthen pandemic preparedness. Future work should explore ddhC dynamics in natural and experimental infections across varying severities and assess its utility in diverse populations and healthcare settings.

Identifiers

PMID40542254
PMCPMC12181374

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.