Evidence mapPaperPMID 40542425Full record

ArticleAlzheimer's research & therapy2025

Delphinidin attenuates cognitive deficits and pathology of Alzheimer's disease by preventing microglial senescence via AMPK/SIRT1 pathway.

Ying Liu, Ting Hong, Mingxuan Lv, Xiaoyu Guo, Panpan Zhang, Aijuan Yan, Wenshi Wei

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In one paragraph

Article in Alzheimer's research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Ying Liu *Department of Neurology, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China.
Ting Hong *Shanghai Key Laboratory of Clinical Geriatric Medicine, Fudan University, Shanghai, 200040, China.
Mingxuan LvDepartment of Neurology, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China.
Xiaoyu GuoShanghai Key Laboratory of Clinical Geriatric Medicine, Fudan University, Shanghai, 200040, China.
Panpan ZhangDepartment of Neurology, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China.
Aijuan YanDepartment of Neurology, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China. yanaijuan1928@163.com.
Wenshi WeiDepartment of Neurology, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China. wenshiwei1994@163.com.

Funding

National Natural Science Foundation of China 82201565National Natural Science Foundation of China 82302417Natural Science Foundation of Shanghai Municipality 24ZR1420800Shanghai Sailing Program 21YF1411600the Investigator Initiated Research Projects of Huadong Hospital HDLC2022003the Qingmiao Talent Program of Huadong Hospital QMRC2203the Shanghai Hospital Development Center Foundation SHDC22022304
6 · The paper itself

Abstract

backgroundEmerging evidence suggests that senescent microglia play a role in β-amyloid (Aβ) pathology and neuroinflammation in Alzheimer's disease (AD). Targeting senescent cells with naturally derived compounds exhibiting minimal cytotoxicity represents a promising therapeutic strategy.

objectivesThis study aimed to investigate whether delphinidin, a naturally occurring anthocyanin, can alleviate AD-related pathologies by mitigating microglial senescence and to elucidate the underlying molecular mechanisms.

methodsWe employed APP/PS1 mice, naturally aged mice, and an in vitro model using Aβ42-induced senescent BV2 microglia. Delphinidin's effects were evaluated through assessments of cognitive function, synaptic integrity (synapse loss), Aβ plaque burden, senescent microglia gene signatures, and cellular senescence markers (including senescence-associated β-galactosidase activity, SASP factor expression, oxidative stress, and cyclin p21/p16 levels). Mechanistic studies involved analyzing the AMPK/SIRT1 signaling pathway, testing direct delphinidin-SIRT1 interaction, and using the AMPK inhibitor Compound C.

resultsDelphinidin treatment significantly alleviated cognitive deficits, synapse loss, Aβ peptides plaques of APP/PS1 mice via downregulated senescent microglia gene signature, prevented cell senescence, including senescence-associated β-galactosidase activity, senescence-associated secretory phenotype (SASP), oxidative stress, cyclin p21 and p16. And delphinidin treatment also prevented microglial senescence in naturally aged mice. In vitro, delphinidin treatment attenuated cell senescence induced by Aβ42 in BV2 microglia cells. Further research indicated that delphinidin treatment enhanced the AMPK/SIRT1 signaling pathway. Additionally, delphinidin was found to directly interact with SIRT1. It's noteworthy that AMPK inhibitor Compound C inversed the protective effect of delphinidin against microglial senescence.

conclusionOur study reveals for the first time that delphinidin effectively improved cognitive deficits, alleviated synapse loss and Aβ pathology in APP/PS1 mice by mitigating microglial senescence. These findings highlight delphinidin as a promising natural anti-aging agent against the development of aging and age-related diseases.

Indexed as

Alzheimer DiseaseAMP-Activated Protein KinasesAnthocyaninsCellular SenescenceCognitive DysfunctionMicrogliaSirtuin 1Amyloid beta-PeptidesAmyloid beta-Protein PrecursorAnimalsDisease Models, AnimalMaleMiceMice, TransgenicPresenilin-1Signal TransductionAMP-Activated Protein KinasesAmyloid beta-PeptidesAmyloid beta-Protein PrecursorAnthocyaninsdelphinidinPresenilin-1Sirt1 protein, mouseSirtuin 1AgingAlzheimer's diseaseAMPKMicroglial senescenceNeuroinflammation

Identifiers

PMID40542425
PMCPMC12180243

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.