ArticleMolecular nutrition & food research2025
Protective Effects of Human Milk Oligosaccharides (hMOs) on Analgesic, Nonsteroidal Anti-inflammatory Drugs (NSAIDs)-Induced Disruption of Gut Barrier Integrity in Cocultures Mimicking Neuro-Gut Epithelial Cell Crosstalk.
Article in Molecular nutrition & food research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Human Milk Oligosaccharide LNnT Attenuates Colonic Barrier Dysfunction and Associated Cognitive Impairment via Modulating Sphingolipid Metabolism and Gut Microbiota.Molecules (Basel, Switzerland) · 2026Article
- Protective Effects of Human Milk Oligosaccharides (hMOs) on Analgesic, Nonsteroidal Anti-inflammatory Drugs (NSAIDs)-Induced Disruption of Gut Barrier Integrity in Cocultures Mimicking Neuro-Gut Epithelial Cell Crosstalk.Molecular nutrition & food research · 2025Article
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4 authors.
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Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs) like indomethacin alter gut microbiota composition and disrupt intestinal integrity, increasing the risks of inflammatory bowel disease (IBD). Gut barrier function is regulated by a complex crosstalk between different cell types, including epithelial cells and cells from the enteric nervous system (ENS). Human milk oligosaccharides (hMOs), particularly 2'-fucosyllactose (2'-FL) and 3-fucosyllactose (3-FL), can regulate intestinal barrier function, but their roles in neuroepithelial crosstalk under NSAID stress remain unclear. This study focused on hMOs' direct epithelial effects to explore their potential in microbiota-independent effects caused by the NSAID indomethacin. T84 colorectal carcinoma cells were cocultured with SH-SY5Y neuroblastoma cells in ratios of 29:1 and 14:1. We evaluated the protective role of hMOs in indomethacin-induced barrier disruption. We found that indomethacin reduced transepithelial electrical resistance (TEER), disrupted F-actin organization, and decreased tight junction (TJ) protein expression. Pretreatment with 2'-FL and 3-FL protected against these effects, particularly in cocultures with a higher nerve cell ratio of 14:1, restoring TEER, F-actin integrity, and claudin-1 expression. These findings highlight the therapeutic potential of 2'-FL and 3-FL in directly maintaining barrier integrity in intestinal neuroepithelial cocultures during NSAID treatment.
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