Evidence mapPaperPMID 40548950Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Metabolic Stress-Induced Choline Kinase α (CHKA) Activation in Endothelial Subpopulation Contributes to Diabetes-Associated Microvascular Dysfunction.

Ling Ren, Linyu Zhang, Yun Bai, Chang Huang, Xiaosa Li, Fanfei Ma, Wan Mu, Mudi Yao, Chang Jiang, Xiangjun Chen and 2 more

Abstract read
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ling RenDepartment of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.
Linyu ZhangThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China.
Yun BaiCollege of Information Science, Shanghai Ocean University, Shanghai, 201306, China.
Chang HuangDepartment of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.
Xiaosa LiDepartment of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.
Fanfei MaThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China.
Wan MuDepartment of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.
Mudi YaoDepartment of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.
Chang JiangThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China.
Xiangjun ChenEye Center of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.
Qin JiangThe Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China.
Biao YanDepartment of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China.ORCID https://orcid.org/0009-0007-0063-5718

Funding

Fundamental Research Funds for the Central Universities YG2025ZD04National Natural Science Foundation of China 82225013Shanghai Municipal Health Commission Collaborative Innovation Cluster Project 2024CXJQ02
6 · The paper itself

Abstract

Diabetes is a prevalent metabolic disorder, and its associated microvascular dysfunction plays a key role in the pathogenesis of complications such as retinopathy, nephropathy, and peripheral vascular disease. However, the mechanism governing metabolic regulation and functional heterogeneity of endothelial dysfunction remains incompletely understood. This study investigates the role of metabolic stress-induced activation of choline kinase α (CHKA) in endothelial cell (EC) subpopulations, contributing to diabetes-induced microvascular dysfunction. Using single-cell RNA sequencing (scRNA-seq), three distinct EC subclusters are identified within retinal vessels. Among them, one subcluster characterized by elevated CHKA expression is associated with enhanced angiogenic activity. CHKA silencing in ECs inhibited angiogenic effects and reduced retinal vascular dysfunction in diabetic murine models. CHKA silencing also disrupted NAD

Indexed as

Choline KinaseDiabetes Mellitus, ExperimentalEndothelial CellsStress, PhysiologicalAnimalsHumansMaleMiceMice, Inbred C57BLRetinal VesselsCholine Kinasediabetic retinopathyendothelial cellsheterogeneitysingle cell RNA sequencing

Identifiers

PMID40548950
PMCPMC12412581

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.