Evidence map›Paper›PMID 40558428›Full record

ArticleBiosensors2025

Multiplexed CRISPR Assay for Amplification-Free Detection of miRNAs.

P I Thilini De Silva, Keshani Hiniduma, Rachelle Canete, Ketki S Bhalerao, Sherif M Shawky, Hansana Gunathilaka, Jessica L Rouge, Islam M Mosa, David C Steffens, Kevin Manning and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

P I Thilini De SilvaDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Keshani HinidumaDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.ORCID 0009-0004-9884-2456
Rachelle CaneteDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.ORCID 0009-0007-8653-3164
Ketki S BhaleraoDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.ORCID 0000-0003-3956-9432
Sherif M ShawkyDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.ORCID 0000-0001-5020-5563
Hansana GunathilakaDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Jessica L RougeDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.ORCID 0000-0002-6051-3735
Islam M MosaDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
David C SteffensDepartment of Psychiatry, UConn Health, Farmington, CT 06030, USA.
Kevin ManningDepartment of Psychiatry, UConn Health, Farmington, CT 06030, USA.
Breno S DinizDepartment of Psychiatry, UConn Health, Farmington, CT 06030, USA.
James F RuslingDepartment of Chemistry, University of Connecticut, Storrs, CT 06269, USA.ORCID 0000-0002-6117-3306

Funding

U.S. National Science Foundation 2231490
6 · The paper itself

Abstract

CRISPR-Cas proteins from bacteria are powerful tools for gene editing and molecular diagnostics. Expanding capacity of CRISPR to low cost, multiplexed assays of biomarkers is a key to future disease diagnostics, since multiple biomarker detection is essential for reliable diagnostics. Herein we describe a multiplexed assay in a 3D-printed 96-well plate with CRISPR-Cas13a immobilized in each well to target three circulating blood biomarker microRNAs (miRNAs 34c-5p, 200c-3p, and 30e-5p) for Alzheimer's disease (ALZ). Immobilized Cas13a is equipped with different crRNAs complementary to each miRNA target. MiRNA binding to crRNA complements activates the collateral RNase activity of Cas13a, cleaving a quenched fluorescent reporter (RNaseAlert) with fluorophore and quencher connected by an RNA oligonucleotide to enable fluorescence measurements. We achieved ultralow limits of detection (LOD) of 0.74 fg/mL for miRNA 34c-5p, 0.70 fg/mL for miRNA 30e-5p, and 7.4 fg/mL for miRNA 200c-3p, with dynamic ranges from LODs up to about 1800 pg/mL. The accuracy of the assay was validated by spike-recovery studies and good correlation of levels of patient plasma samples vs. a referee method. This new approach provides selective, sensitive multiplex miRNA biosensing, and simultaneously accommodates analysis of standards and controls.

Indexed as

Biosensing TechniquesCRISPR-Cas SystemsMicroRNAsHumansLimit of DetectionMicroRNAsAlzheimer biomarkersCRISPRfluorescencemiRNAsmultiplexed assay

Identifiers

PMID40558428
PMCPMC12190982

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.