Evidence map›Paper›PMID 40559771›Full record

ArticleVeterinary sciences2025

The Effect of CDKN1A on the Expression of Genes Related to Milk Protein and Milk Fat Synthesis in Bovine Mammary Epithelial Cells.

Yuanyuan Zhang, Junxi Liang, Kai Zhang, Hong Su, Daqing Wang, Min Zhang, Feifei Zhao, Zhiwei Sun, Zhimin Wu, Guifang Cao and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yuanyuan ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.ORCID 0009-0003-3791-8296
Junxi LiangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Kai ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.ORCID 0000-0001-5235-4562
Hong SuCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.ORCID 0000-0002-8656-8259
Daqing WangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Min ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Feifei ZhaoCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhiwei SunTongliao Institute of Agricultural and Animal Husbandry Sciences, Tongliao 028000, China.
Zhimin WuCollege of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
Guifang CaoTongliao Institute of Agricultural and Animal Husbandry Sciences, Tongliao 028000, China.
Yong ZhangCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China.

Funding

the "Jiebangguashuai" project of the Inner Mongolia Autonomous Region, China No. 2022JBGS0021
6 · The paper itself

Abstract

Milk fat is an important nutritional component and flavor substance in dairy products. Its content and composition directly affect the nutritional value, processing characteristics, and economic benefits of dairy products. Therefore, an in-depth exploration of the molecular mechanisms that influence milk protein synthesis holds profound significance for dairy farming and dairy production. Molecular biology techniques were used to construct CDKN1A overexpression and interference vectors. Using BMECs (bovine mammary epithelial cells) as the experimental model, the vectors were transfected into the cells via liposome mediation to investigate the effect of CDKN1A on the expression of genes related to milk protein synthesis. The results showed that the CDKN1A overexpression and interference vectors were successfully constructed, and the overexpression of CDKN1A reduced milk protein synthesis, and the interference of CDKN1A enhanced milk protein synthesis. This finding provides an important theoretical basis for dairy farming and dairy production. By regulating the expression level of CDKN1A, it is possible to achieve precise control of milk protein yield in dairy cows. It also offers a potential target for the development of new feed additives or drugs. These additives or drugs can promote milk protein synthesis by regulating the activity of CDKN1A, providing new strategies and methods for the sustainable development of the dairy industry.

Indexed as

bovine mammary epithelial cellsCDKN1Aexpression vector constructionmilk fat synthesismilk protein synthesis

Identifiers

PMID40559771
PMCPMC12197750

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.