Evidence mapPaperPMID 40559986Full record

ArticleToxics2025

Bioassay Using the DR-EcoScreen System to Measure Dioxin-Related Compounds in Serum Samples from Individuals Exposed to Dioxins Originating from Agent Orange in Vietnam.

Masafumi Nakamura, Ryo Matsuda, Hoa Thi Vu, Tai Pham-The, Thao Ngoc Pham, Tomoya Takiguchi, Hisao Nishijo, Muneko Nishijo

Abstract read
In one paragraph

Article in Toxics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Masafumi NakamuraHiyoshi Corporation, Omihachiman 523-8555, Japan.
Ryo MatsudaHiyoshi Corporation, Omihachiman 523-8555, Japan.
Hoa Thi VuDepartment of Military Hygiene, Vietnam Military Medical University, Hanoi 12108, Vietnam.ORCID 0009-0002-4863-0653
Tai Pham-TheBiomedical and Pharmaceutical Research Centre, Vietnam Military Medical University, Hanoi 12108, Vietnam.ORCID 0000-0002-5878-7307
Thao Ngoc PhamDepartment of Functional Diagnosis, Military Hospital 103, Vietnam Military Medical University, Hanoi 12108, Vietnam.
Tomoya TakiguchiEpidemiology and Public Health, Kanazawa Medical University, Uchinada 920-0293, Japan.ORCID 0000-0001-7979-3813
Hisao NishijoDepartment of Sport and Health Sciences, Faculty of Human Sciences, University of East Asia, Shimonoseki 751-8503, Japan.ORCID 0000-0002-4786-4610
Muneko NishijoEpidemiology and Public Health, Kanazawa Medical University, Uchinada 920-0293, Japan.

Funding

the Ministry of Education, Sports, Science and Culture, Japan 17H04665 and 18K19709
6 · The paper itself

Abstract

A bioassay for dioxin analysis of human samples has the advantages of cost effectiveness and requiring only a small sample volume. Using a DR-EcoScreen bioassay, we measured the biological equivalency (BEQ) levels in serum samples from 32 men exposed to dioxins in Bien Hoa and 32 unexposed men in Hanoi, Vietnam. For the Bien Hoa men, the World Health Organization toxic equivalent (WHO-TEQ) levels of dioxins had already been measured by instrumental analysis. The difference in fat-based BEQ levels between exposed and unexposed men was greater than for crude BEQ levels, with a strong correlation between fat-based BEQ and WHO-TEQ levels. The fat-based BEQ levels in Bien Hoa men with longer residency but lower WHO-TEQ levels were significantly higher than those in unexposed men and Bien Hoa men with shorter residency but higher WHO-TEQs, suggesting that fat-based BEQ may be an effective marker of dioxin-like activity. Additionally, comparisons of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and TEQs between shorter- and longer-residency groups indicated that higher levels of polychlorinated dibenzo-p-dioxins (PCDDs), particularly TCDD, contribute to increased BEQ levels. Taken together, the DR-EcoScreen bioassay may be useful to analyze dioxin-like activity associated with WHO-TEQs of men in a dioxin contamination hotspot originating from Agent Orange in Vietnam.

Indexed as

bioassaybiological equivalencydioxinsserum samplesthe World Health Organization toxic equivalentVietnamese men

Identifiers

PMID40559986
PMCPMC12197712

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.