Evidence mapPaperPMID 40564910Full record

ArticleInternational journal of molecular sciences2025

The Design of a Multistage Monitoring Protocol for Dendritic Cell-Derived Exosome (DEX) Immunotherapy: A Conceptual Framework for Molecular Quality Control and Immune Profiling.

Ramón Gutiérrez-Sandoval, Francisco Gutiérrez-Castro, Natalia Muñoz-Godoy, Ider Rivadeneira, Adolay Sobarzo, Luis Alarcón, Wilson Dorado, Andy Lagos, Diego Montenegro, Ignacio Muñoz and 5 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Ramón Gutiérrez-SandovalDepartment of Oncopathology, OGRD Alliance, Lewes, DE 19958, USA.ORCID 0009-0004-8428-9549
Francisco Gutiérrez-CastroDepartment of Cancer Research, Flowinmunocell-Bioexocell Group, 08028 Barcelona, Spain.
Natalia Muñoz-GodoyDepartment of Cancer Research, Flowinmunocell-Bioexocell Group, 08028 Barcelona, Spain.
Ider RivadeneiraDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Adolay SobarzoDepartamento de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad San Sebastián, Lientur 1457, Concepción 4080871, Chile.
Luis AlarcónDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Wilson DoradoDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Andy LagosDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Diego MontenegroDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Ignacio MuñozDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Rodrigo AguileraDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Jordan IturraDepartment of Outreach and Engagement Programs for OGRD Consortium, Charlestown KN0802, Saint Kitts and Nevis.
Francisco KrakowiakDepartment of Molecular Oncopathology, Bioclas, Concepción 4030000, Chile.
Cristián Peña-VargasDepartment of Oncopathology, OGRD Alliance, Lewes, DE 19958, USA.
Andrés ToledoDepartment of Oncopathology, OGRD Alliance, Lewes, DE 19958, USA.

Funding

Fundación Biotech FB-20222-0871
6 · The paper itself

Abstract

The increasing complexity of dendritic cell (DC)-derived exosome (DEX) immunotherapy demands structured monitoring protocols capable of translating molecular activity into actionable clinical outputs. This study proposes a standardized, multistage immunomonitoring framework designed to evaluate immune activation, cytokine polarization, and product integrity in DEX-based therapies. The protocol integrates open access methodologies-flow cytometry, cytometric bead array (CBA), and Western blotting-to assess CD69/CD25 activation, Th1/Th2/Th17 cytokine profiles, and vesicle identity across distinct checkpoints. These outputs are consolidated within the Structured Immunophenotypic Traceability Platform (STIP), which applies logic-based classifications (Type I-III) to support reproducible stratification of immune responses. Functional validation was performed through ex vivo co-culture models, enabling real-time interpretation of immune polarization, cytotoxic potential, and batch consistency. These outputs are supported by previous experimental validations published in

Indexed as

Dendritic CellsExosomesImmunotherapyCytokinesFlow CytometryHumansImmunophenotypingQuality ControlCytokinescytokine logic ratiosdendritic cell-derived exosomesex vivo immune profilingnon-pharmacodynamic platformphenotypic immune classificationSTIPstructured immunomonitoringvesicle-based quality control

Identifiers

PMID40564910
PMCPMC12193272

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.