Evidence mapPaperPMID 40565062Full record

ArticleInternational journal of molecular sciences2025

Metabolic Reprogramming of Urothelial Carcinoma-A Theragnostic Target for Betulinic Acid.

Anirban Ganguly, Aratrika Halder, Keara Healy, Stephanie Daugherty, Shingo Kimura, Rajkumar Banerjee, Jonathan M Beckel, Pradeep Tyagi

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anirban GangulyDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Aratrika HalderOils, Lipids Science & Technology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India.
Keara HealyDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Stephanie DaughertyDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Shingo KimuraDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.ORCID 0000-0003-2164-3091
Rajkumar BanerjeeOils, Lipids Science & Technology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India.ORCID 0000-0003-2336-6294
Jonathan M BeckelDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.ORCID 0000-0003-1390-2292
Pradeep TyagiDepartment of Urology, University of Pittsburgh School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA.ORCID 0000-0001-6586-4545

Funding

NCI NIH HHS R21 CA263243NIDDK NIH HHS R01 DK117884NIH HHS 1R21CA263243-22A1
6 · The paper itself

Abstract

A pivotal role of metabolic reprogramming in urothelial carcinoma is hallmarked by the dependence of two-fold faster proliferation of urothelial carcinoma cell line T24 than benign cell line TRT-HU1 on five-fold higher glucose (basal) 16 mM vs. 3 mM in McCoy's 5A media and Keratinocyte Serum Free media, respectively. Here, we report that an additional 10% increase to 17.6 mM and 3.3 mM glucose significantly shortens the doubling time by 3 h and 1 h for T24 and TRT-HUI, respectively. T24 grown at 17.6 mM glucose lowers the confocal localization of the fatty acid mimetic, Betulinic Acid (BA) conjugated to FITC (BA-FITC) with Mito Tracker Red (mitochondrial marker), which doubles the IC50 of BA and BA-FITC by lowering cell cycle arrest in the G0/G1 phase from 54.2% to 43.8% and caspase-3/7 mediated apoptosis and by reversing

Indexed as

TriterpenesUrinary Bladder NeoplasmsUrotheliumApoptosisBetulinic AcidCell Line, TumorCell ProliferationGlucoseHumansMetabolic ReprogrammingPentacyclic TriterpenesBetulinic AcidGlucosePentacyclic TriterpenesTriterpenesbetulinic acidmetabolic reprogrammingmitomycin Ctheragnosticurothelial carcinoma

Identifiers

PMID40565062
PMCPMC12192694

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.